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Anti-GPR50 Antibody

G Protein-Coupled Receptor 50, Melatonin-Related Receptor

Cat #: AGR-061
Alternative Name G Protein-Coupled Receptor 50, Melatonin-Related Receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)ESFRREYWTIFHAMR, corresponding to amino acid residues 307 - 321 of mouse GPR50 (Accession O88495). Intracellular, C-terminus.
Accession (Uniprot) Number O88495
Gene ID 14765
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - identical, Human - 14 out of 15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1-2. Anti-GPR50 Antibody (#AGR-061), (1:200).
    3-4. Anti-GPR50 Antibody, preincubated with GPR50 Blocking Peptide (BLP-GR061).
References
  1. Trzaskowski et al (2012), Current Medicinal Chemistry, 19, 1090.
  2. Grünewald, et al (2012), ACS Chem Neurosci., 20, 459.
  3. Khan et al (2016), Biomed Pharmacother, 78, 121.
  4. Ahmad et al (2018), Oncoscience., 5, 157.
Scientific background

G protein-coupled receptor 50 (GPR50), previously known as the melatonin-related receptor, share 45% sequence identity to melatonin receptors MT1 and MT2 but it does not bind melatonin or any other known ligand and therefore remains an orphan receptor without a clear function1,2.

GPR50 belongs to the G protein coupled receptors (GPCRs) family, a large group of receptors characterized by seven trans membrane helices that transmit a signal from the outside cellular surface into the inside of the cell through conformational changes. GPCRs mediate most of the physiological responses to hormones, neurotransmitters and environmental stimulants, and so have great potential as therapeutic targets for a broad spectrum of diseases1.

GPR50 was identified as a risk gene for bipolar disorder and major depression and it was shown to have a role in neural development and sterol metabolism, it was also shown to be related to mood disorders, adaptive thermogenesis and torpor response (the body's response to starvation)2.

 In addition, it was shown to have a dramatic effect on neurite outgrowth, supporting a likely role in neural development and it was also found to be expressed in various types of cancer3.

GPR50 is expressed in the pituitary, hypothalamus, cortex, midbrain, pons, amygdala, and in several brainstem nuclei3,4

Due to its involvement in a wide range of mental conditions and disease, GPR50 is a major target for research and drug development.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/07/2021

Anti-GPR50 Antibody (#AGR-061) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot applications. It has been designed to recognize GPR50 from mouse, rat and human samples.

For research purposes only, not for human use
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