- Peptide (C)ESFRREYWTIFHAMR, corresponding to amino acid residues 307 - 321 of mouse GPR50 (Accession O88495). Intracellular, C-terminus.
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-GPR50 Antibody (#AGR-061), (1:200).
3-4. Anti-GPR50 Antibody, preincubated with GPR50 Blocking Peptide (BLP-GR061).
- Trzaskowski et al (2012), Current Medicinal Chemistry, 19, 1090.
- Grünewald, et al (2012), ACS Chem Neurosci., 20, 459.
- Khan et al (2016), Biomed Pharmacother, 78, 121.
- Ahmad et al (2018), Oncoscience., 5, 157.
G protein-coupled receptor 50 (GPR50), previously known as the melatonin-related receptor, share 45% sequence identity to melatonin receptors MT1 and MT2 but it does not bind melatonin or any other known ligand and therefore remains an orphan receptor without a clear function1,2.
GPR50 belongs to the G protein coupled receptors (GPCRs) family, a large group of receptors characterized by seven trans membrane helices that transmit a signal from the outside cellular surface into the inside of the cell through conformational changes. GPCRs mediate most of the physiological responses to hormones, neurotransmitters and environmental stimulants, and so have great potential as therapeutic targets for a broad spectrum of diseases1.
GPR50 was identified as a risk gene for bipolar disorder and major depression and it was shown to have a role in neural development and sterol metabolism, it was also shown to be related to mood disorders, adaptive thermogenesis and torpor response (the body's response to starvation)2.
In addition, it was shown to have a dramatic effect on neurite outgrowth, supporting a likely role in neural development and it was also found to be expressed in various types of cancer3.
GPR50 is expressed in the pituitary, hypothalamus, cortex, midbrain, pons, amygdala, and in several brainstem nuclei3,4.
Due to its involvement in a wide range of mental conditions and disease, GPR50 is a major target for research and drug development.
Species reactivity key:
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Anti-GPR50 Antibody (#AGR-061) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot applications. It has been designed to recognize GPR50 from mouse, rat and human samples.