Anti-GPR91 (SUCNR1) (extracellular) Antibody

G-protein coupled receptor 91, Succinate receptor 1
  • New
Cat #: ASR-090
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)RNVRIASRLDSWPQG, corresponding to amino acid residues 248-262 of rat GPR91/Succinate Receptor 1 (Accession Q6IYF9). 3rd extracellular loop.
    Accession (Uniprot) Number Q6IYF9
    Gene ID 408199
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – 14/15 amino acid residues identical; human – 12/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic, ih, ip, lci
    Western blot
    Western blot analysis of rat kidney membrane (lanes 1 and 3), mouse kidney lysate (lanes 2 and 4), mouse TK-1 T-cell lymphoma (lanes 5 and 8), mouse J774 macrophage cell (lanes 6 and 9) and human Jurkat acute T cell leukemia (lanes 7 and 10) lysates:
    1,2,5,6,7. Anti-GPR91 (SUCNR1) (extracellular) Antibody (#ASR-090) (1:200).
    3,4,8,9,10. Anti-GPR91 (SUCNR1) (extracellular) Antibody, preincubated with the control peptide antigen.
    Indirect flow cytometry
    Indirect flow cytometry of live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + Goat anti-rabbit-FITC.
    ___ Cells + Anti- GPR91 (SUCNR1) (extracellular) Antibody (#ASR-090), (2.5 μg/0.5x106 cells).
    References
    1. Lu, Y.T. et al. (2018) Cell Death Dis. 9, 672.
    2. Cherif, H. et al. (2018) PLoS Biol. 16, e2003619.
    3. Le, C.T. et al. (2018) PLoS One 13, e0192146.
    Scientific background

    GPR91 (Succinate Receptor 1), a member of the GPCR superfamily, is specifically activated by extracellular succinate outside the tricarboxylic acid cycle, resulting in activation of paracrine and endocrine effectors. Activation of GPR91 is associated with several diseases, including hypertension and diabetes1,2.

    GPR91 was shown to regulate normal retinal vascularization, proliferative ischemic retinopathy and cortical revascularization post-ischemia. Activation of GPR91 influences different tissues such as kidney, heart, retina, white adipose tissue, and liver.

    Through the activation of GPR91, succinate affects motility, migration, and growth of dendritic cells. In addition, the receptor directly promotes chemotaxis and potentiates activation initiated by Toll-like receptor agonists in dendritic cells1-3.

    In the heart GPR91 mediates succinate-induced cardiomyocyte death. It was demonstrated that upholding levels of serum succinate can cause cardiac hypertrophy through the activation of the GPR911,2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 15/11/2018

    Anti-GPR91 (SUCNR1) (extracellular) Antibody (#ASR-090) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and live cell flow cytometry. It has been designed to recognize GPR91 from rat, mouse, and human samples.

    For research purposes only, not for human use