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Anti-Human BLT1 (extracellular) Antibody

Leukotriene B4 receptor 1, LTB4R, LTB4R1, CMKRL1, GPR16, P2Y7, P2RY7

Cat #: ALR-001
Alternative Name Leukotriene B4 receptor 1, LTB4R, LTB4R1, CMKRL1, GPR16, P2Y7, P2RY7
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h
Immunogen
  • Peptide CFPRYPSEGHRAFH, corresponding to amino acid residues 168-181 of human LTB4R (Accession Q15722). 2nd extracellular loop.
Accession (Uniprot) Number Q15722
Gene ID 1241
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Monkey - identical.
RRID AB_10658484.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Not recommended for use with rat and mouse samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*, lci*
Western blot
  • Western blot analysis of acute T-cell leukemia Jurkat (lanes 1 and 4), promyelocytic leukemia HL-60 (lanes 2 and 5) and acute monocytic leukemia THP-1 (lanes 3 and 6) human cell lysates:
    Western blot analysis of acute T-cell leukemia Jurkat (lanes 1 and 4), promyelocytic leukemia HL-60 (lanes 2 and 5) and acute monocytic leukemia THP-1 (lanes 3 and 6) human cell lysates:
    1-3. Anti-Human BLT1 (extracellular) Antibody (#ALR-001), (1:200).
    4-6. Anti-Human BLT1 (extracellular) Antibody, preincubated with Human BLT1 (extracellular) Blocking Peptide (#BLP-LR001).
Indirect flow cytometry
  • Cell surface detection of LTB4R in live intact human T cell leukemia (Jurkat) cell line:
    Cell surface detection of LTB4R in live intact human T cell leukemia (Jurkat) cell line:
    ___ Control cells + goat-anti-rabbit-Cy5.
    ___ Cells + Anti-Human BLT1 (extracellular) Antibody (#ALR-001), (1:25) + goat-anti-rabbit-Cy5.
  • Cell surface detection of LTB4R in live intact human acute monocytic leukemia (THP-1) cell line:
    Cell surface detection of LTB4R in live intact human acute monocytic leukemia (THP-1) cell line:
    ___ Control cells + goat-anti-rabbit-Cy5.
    ___ Cells + Anti-Human BLT1 (extracellular) Antibody (#ALR-001), (1:25) + goat-anti-rabbit-Cy5.
  • The blocking peptide is not suitable for this application.
References
  1. Riccioni, G. et al. (2008) J. Leukoc. Biol. 84, 1374.
  2. Tager, A.M. and Luster A.D. (2003) Prostaglandins Leukot. Essent. Fatty Acids 69, 123.
  3. Yokomizo, T. et al. (1997) Nature 387, 620.
  4. Owman, C. et al. (1996) Genomics 37, 187.
  5. Yokomizo, T. et al. (2000) J. Exp. Med. 192, 421.
  6. Tryselius, Y. et al. (2000) Biochem. Biophys. Res. Commun. 274, 377.
  7. Kamohara, M. et al. (2000) J. Biol. Chem. 275, 27000.
  8. Wang, S. et al. (2000) J. Biol. Chem. 275, 39482.
Scientific background

Leukotrienes (LTs) are lipid mediators prominently exerting proinflammatory responses. They are divided in two classes: cysteinyl LTs (cysLTs) which have a thioester linkage and LTB41. LTB4, a chemoattractant, plays a prominent role in the recruitment and activation of leukocytes2.

LTB4 acts through two receptors which belong to the G-protein coupled receptor superfamily: BLT1 and BLT2. BLT1 displays high affinity for LTB4 and is highly expressed in leukocytes including granulocytes, macrophages, mast cells, dendritic cells and effector T cells3 and is expressed to much lower levels in the spleen, thymus, heart, skeletal muscle, brain and liver2-4. BLT2 on the other hand is a low affinity receptor which does not display selectivity towards LTB45. Its expression is much more ubiquitous than that of BLT1. It is expressed in most human tissues, with the highest expression being in the spleen, liver, ovary, and leukocytes2,5-8. The two receptors could couple and activate different G-proteins, depending on the cell type and the cellular events activating the receptors2. However, activation of BLT1 usually correlates with increasing intracellular concentrations of Ca2+.

The LTB4/BLT1 system is involved in many allergic reactions as well as asthma induced as an allergic response, where it plays a significant role in recruiting neutrophils and effector T cells into lungs as part of an inflammatory response induced by allergens1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 22/05/2022

Anti-Human BLT1 (extracellular) Antibody (#ALR-001) is a highly specific antibody directed against an extracellular epitope of the human Leukotriene B4 receptor 1. The antibody can be used for western blot and indirect flow cytometry applications. It has been designed to recognize LTB4R from human samples only.

For research purposes only, not for human use
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