Anti-Human CXCR5 (extracellular) Antibody

C-X-C chemokine receptor type 5, Burkitt lymphoma receptor 1, Monocyte-derived receptor 15, MDR-15, CD185
    Cat #: ACR-015
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h
    Immunogen
    Peptide RLDNYNDTSLVENHLC, corresponding to amino acid residues 23-38 of human CXCR5 (Accession P32302). Extracellular, N-terminus.
    Accession (Uniprot) Number P32302
    Gene ID 643
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human only.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity Will not recognize rat and mouse CXCR5.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ifc, lci, wb
    May also work in: ih, ip
    Western blot
    Western blot analysis of Burkitt's lymphoma (Raji) (lanes 1 and 4), human prostate carcinoma (LNCaP) (lanes 2 and 5) and human prostate carcinoma (PC-3) cell line lysates:
    1-3. Anti-Human CXCR5 (extracellular) Antibody (#ACR-015), (1:400).
    4-6. Anti-Human CXCR5 (extracellular) Antibody preincubated with the control peptide antigen.
    Indirect flow cytometry
    Indirect flow cytometry analysis of human promyelocytic leukemia (HL-60) live intact cells:
    ___ Unstained cells.
    ___ Cells + Anti-Human CXCR5 (extracellular) Antibody (#ACR-015), (5 μg/0.5x106 cells).
    Live cell imaging / Immunocytochemistry
    Expression of CXCR5 in human LNCaP cells
    Immunocytochemical staining of intact living human prostate carcinoma (LNCaP) cells. A. Extracellular staining of cells using Anti-Human CXCR5 (extracellular) Antibody (#ACR-015), (green), (1:25) merged with live view of the cells (magnitude X40). B. Extracellular staining of LNCaP cells merged with live view of the cells (magnitude X100).
    References
    1. Murphy, P.M. et al. (2000) Pharmacol. Rev. 52, 145.
    2. Legler, D.F. et al. (1998) J. Exp. Med. 187, 655.
    3. Forster, R. et al. (1996) Cell. 87, 1037.
    Scientific background

    Chemokine receptors are a family of receptors that signal upon binding of one or more members of the chemokine superfamily of chemotactic cytokines. Chemokines coordinate development, differentiation and trafficking of various immune system cells making them key players of the innate and adaptive immune response.

    CXCR5 (also known as Burkitt lymphoma receptor 1) belongs to the CXC subfamily of chemokine receptors and is a G-coupled protein receptor with 7 transmembrane segments. There is considerable homology between CXCR5 and other members of the chemokine receptor family such as IL-9 receptor, CCR-1 and BLR2. CXCR5 expression is detected in peripheral blood CD4 and CD8 leukocytes as well as in secondary lymphatic organs and in granule and Purkinje layers in the brain. It is also expressed in Burkitt’s lymphoma cells1.

    CXCR5 is activated by B-cell attracting chemokine 1 (BCA-1) that is chemotactic for human B lymphocytes. BCA-1 does not affect IL-2 stimulated T-cells, monocytes or neutrophils2. CXCR5 mediates correct T cell localization within granulomas, efficient macrophage activation and lymphoid follicle formation3.

    CXCR5 knockout mice have a severe defect in normal B cell migration and localization. Knockout mice lack inguinal lymph nodes, have a reduced amount of Peyer’s patches and have abnormal primary lymphoid follicles and no functional germinal centers in the spleen4. Absence of CXCR5 has been implicated in increased susceptibility to tuberculosis to due defective T cell localization within the lung3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 04/11/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human CXCR5. Anti-CXCR5 (extracellular) Antibody (#ACR-015) can be used in western blot, immunocytochemistry, and indirect flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize CXCR5 from human samples only.

    For research purposes only, not for human use