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Anti-Human FPR2/ALX (extracellular) Antibody

N-formyl peptide receptor 2, FMLP-related receptor I, FMLPX, Formyl peptide receptor-like 1, FPRL1, ALXR, Lipoxin A4 receptor, LXA4 receptor, LXA4R, RFP

Cat #: AFR-002
Alternative Name N-formyl peptide receptor 2, FMLP-related receptor I, FMLPX, Formyl peptide receptor-like 1, FPRL1, ALXR, Lipoxin A4 receptor, LXA4 receptor, LXA4R, RFP
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h
Immunogen
  • Peptide (C)GGTPEERLKVAIT, corresponding to amino acid residues 184-196 of human FPR2/ALX (Accession P25090). 2nd extracellular loop.
Accession (Uniprot) Number P25090
Gene ID 2358
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human only.
RRID AB_10659349.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.6 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ifc, ih, wb
May also work in: ic*, ip*, lci*
Western blot
  • Western blot analysis of HL-60 (lanes 1 and 3) and K562 (lanes 2 and 4) human leukemia cell lysates:
    Western blot analysis of HL-60 (lanes 1 and 3) and K562 (lanes 2 and 4) human leukemia cell lysates:
    1,2. Anti-Human FPR2/ALX (extracellular) Antibody (#AFR-002), (1:200).
    3,4. Anti-Human FPR2/ALX (extracellular) Antibody, preincubated with Human FPR2/ALX (extracellular) Blocking Peptide (#BLP-FR002).
Immunohistochemistry
  • Human minor salivary glands (1:200) (Nelson, J.W. et al. (2014) Am. J. Physiol. 306, C178.).
Indirect flow cytometry
  • Cell surface detection of FPR2 in live intact chronic myelogenous leukemia (K562) cell line:
    Cell surface detection of FPR2 in live intact chronic myelogenous leukemia (K562) cell line:
    ___ Unstained cells + goat-anti-rabbit-PE.
    ___ Cells + Anti-Human FPR2/ALX (extracellular) Antibody (#AFR-002), (1:20) + goat-anti-rabbit-PE.
  • The blocking peptide is not suitable for this application.
References
  1. Ye, R.D. et al. (2009) Pharmacol. Rev. 61, 119.
  2. Le, Y. et al. (2002) Trends Immunol. 23, 541.
  3. Murphy, P.M. et al. (1992) J. Biol. Chem. 267, 7637.
  4. Becker, E.L. et al. (1998) Cell Tissue Res. 292, 129.
Scientific background

Chemotactic factors from both Gram-positive and Gram-negative bacteria are short peptides with N-formyl methionine at the N-terminus (extensively reviewed in reference 1). These peptides are released from bacteria during infection and activate formyl peptide receptors (FPR), members of the G-protein coupled receptor (GPCR) superfamily. In humans, the FPR family consists mainly of three receptors, FPR1, FPR2/ALX (formerly FPRL1), and FPR3 (formerly FPRL2) which all couple to the Gi subtype of G-proteins and ultimately lead to the activation of phospholipase C and intracellular Ca2+ increase1,2.

FPRL1, or FPR2/ALX as it is commonly called, is a seven transmembrane protein like all GPCRs. This receptor was originally cloned by screening a HL60 neutrophil cDNA library with a FPR1 cDNA probe3. FPR2/ALX shares 69% identity with FPR1 and despite its high homology, it displays relatively low affinity for fmlf, the most potent N-formyl peptide released by bacteria3.

FPR1 was originally found in neutrophils and later found to be distributed in myeloid and non-myeloid cells as is the case for FPR2/ALX and FPR3 (FPR3 though is not expressed in neutrophils). FPR1 is also expressed in multiple organs and tissues including epithelial cells in organs with secretory functions, endocrine cells, liver hepathocytes, smooth muscle cells and endothelial cells, brain spinal cord and both motor and sensory neurons4. FPR2/ALX has a similar tissue distribution to that of FPR1.

While N-formyl peptides were the first peptides found to activate these receptors, the ligand diversity for FPR has proven to be quite broad and demonstrates to be both pro- and anti-inflammatory. They include peptidic ligands originating from bacterial and viral sources (including HIV), endogenous ligands such as chemokines and annexins, short peptides associated with inflammation and infection. Interestingly, β-amyloid peptide, a known marker for Alzheimer’s disease activates FPR2/ALX thereby directly linking these receptors to the neurodegenerative disease. Lipoxin A4 (LXA4) is the first identified endogenous ligand for FPR2/ALX and is an eicosanoid with potent anti-inflammatory characteristics1.

Considering that FPR2/ALX can be activated by two different types of ligands, this receptor can be categorized in two different GPCR subgroups: Formyl peptide receptors and Leukotriene receptors.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title:

Anti-Human FPR2/ALX (extracellular) AntibodyExpression of FPR2/ALX in human minor salivary glands.Immunohistochemical staining of human minor salivary gland (hMSG) sections using Anti-Human FPR2/ALX (extracellular) Antibody (#AFR-002). FPR2/ALX staining (green) is localized on the apical side of acinar and ductal hMSG. Phalloidin (red) is used to stain actin filaments and prodium iodide is used to stain nuclei. Adapted from Nelson, J.W. et al. (2014) Am. J. Physiol. 306, C178. with permission of The American Physiological Society.

Last update: 11/04/2021

Anti-Human FPR2/ALX (extracellular) Antibody (#AFR-002) is a highly specific antibody directed against an epitope of the human N-formyl peptide receptor 2. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize FPRL2/ALX from human samples only.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Published figures using this product
  • Expression of ALX/FPR2 in hMSG.
    Expression of ALX/FPR2 in hMSG.
    Immunohistochemical staining of human fixed frozen minor salivary gland sections using Anti-Human FPR2/ALX (extracellular) Antibody (#AFR-002). A. Merged image of FPR2 staining (green) with propidium iodide. B. FPR2 staining (green) is strongly localized on the apical side of acinar and ductal hMSG. C. Cell nuclei are stained with propidium iodide (blue). D. Labeled phalloidin is used to label actin filaments. E. Merged image of B, C and D.
    Adapted from Nelson, J.W. et al. (2014) with permission of the American Physiological Society.
Immunohistochemistry citations
  1. Human minor salivary glands (1:200).
    Nelson, J.W. et al. (2014) Am. J. Physiol. 306, C178.
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