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Anti-Human TRPM1 Antibody

Melastatin-1, MLSN1, LTrpC1, Transient receptor potential cation channel subfamily M member 1

Cat #: ACC-041
Alternative Name Melastatin-1, MLSN1, LTrpC1, Transient receptor potential cation channel subfamily M member 1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h
Immunogen
  • Peptide (C)EEKKVKKEKASTETE, corresponding to amino acid residues 1518-1533 of human TRPM1 (Accession O75560). Intracellular, C-terminus.
Accession (Uniprot) Number O75560
Gene ID 4308
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Canis - 12/16 amino acid residues identical.
RRID AB_2040245.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Will not recognize rat or mouse TRPM1.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of A875 (human melanoma cell line) lysate:
    Western blot analysis of A875 (human melanoma cell line) lysate:
    1. Anti-Human TRPM1 Antibody (#ACC-041), (1:200).
    2. Anti-Human TRPM1 Antibody, preincubated with Human TRPM1 Blocking Peptide (#BLP-CC041).
References
  1. Fleig, A. et al. (2004) Trends. Pharmacol. Sci. 25, 633.
  2. Vazquez, E. et al. (2006) Semin. Cell. Dev. Biol. 17, 607.
  3. Kraft, R. et al. (2005) Pflugers Arch. 451, 204.
  4. Fang, D. et al. (2000) Biochem. Biophys. Res. Commun. 279, 53.
Scientific background

The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after  the first discovered member melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas. 

The TRPM family consist of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM7.

The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N-terminus and C-terminus. However, due to their  long N-terminus and C-terminus they were also named the long TRP channel family. 

TRPM channels were suggested to play a role in tumourigenesis, proliferation and differentiation.

Recently, TRPM1 was found to be down-regulated in a highly metastatic line. The loss of TRPM1 expression has been reported to be correlated to melanoma aggressiveness.

Two alternative splice variants of TRPM1 were reported; short (TRPM1-S) and long (TRPM1-L). The TRPM1-S was described to be expressed at human melanocyte and mainly in metastasic melanoma cell lines. While TRPM1-L is expressed at murine CNS.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 05/09/2021

Anti-Human TRPM1 Antibody (#ACC-041) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize TRPM1 from human samples only.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

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