Anti-Human Tumor Necrosis Factor Receptor 1 (extracellular)

TNFRSF1A, TNF Receptor Superfamily Member 1A, TNFR1, CD120a, Tumor Necrosis Factor Receptor Type 1
    Cat #: ANT-041
    Sizes: 25 µl, 50 µl, 0.2 ml
  • Lyophilized Powder
  • Antigen Incl.
  • QC Tested
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h
    Immunogen
    Peptide CRKNQYRHYWSEN, corresponding to amino acid residues 127 - 139 of human TNF Receptor 1 (Accession P19438). Extracellular, N-terminus
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology The antibody recognizes only human TNF Receptor 1.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity This antibody will also recognize the soluble form of human TNF Receptor 1 (also known as Tumor Necrosis Factor Binding Protein 1).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic*, ih*, ip*, lci*
    Western blot
    Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate:
    1. Anti-Human Tumor Necrosis Factor Receptor 1 (extracellular) antibody (#ANT-041), (1:200).
    2. Anti-Human Tumor Necrosis Factor Receptor 1 (extracellular) antibody, preincubated with the control peptide antigen.
    Western blot analysis of human PANC-1 pancreas ductal adenocarcinoma cell line lysate:
    1. Anti-Human Tumor Necrosis Factor Receptor 1 (extracellular) antibody (#ANT-041), (1:200).
    2. Anti-Human Tumor Necrosis Factor Receptor 1 (extracellular) antibody, preincubated with the control peptide antigen.
    Indirect flow cytometry
    Indirect flow cytometry of live intact human MEG-01 megakaryoblastic leukemia cells:
    ___ Cells.
    ___ Cells + Goat-anti-Rabbit-FITC.
    ___ Cells + Anti-Human Tumor Necrosis Factor Receptor 1 (extracellular) antibody (#ANT-041), 5 µg + Goat-anti-Rabbit-FITC.
    References
    1. Bradshaw, R.A. and Dennis, E.A. (2009) Handbook of Cell Signaling Volume 1, Second edition.
    2. Li, J. et al. (2013) Adv. Immunol. 119, 135.
    3. Sedger, L.M. and Mcdermott, M.F. (2014) Cytokine Growth Factor Rev. 25, 453.
    Scientific background

    TNF (Tumor necrosis factor) has a wide range of biological effects in host defense against pathogens. It is capable of inducing cell survival, proliferation and differentiation as well as both apoptotic and necrotic cell death under certain conditions. The TNFR1 receptor belongs to a family of type I transmembrane proteins.
    TNFR1, as well as other members of its family, is an elongated molecule composed of an extracellular domain of multiple ~40-residue pseudo repeats, with six cysteines forming three disulfide bridges. These modules are termed CRDs (cysteine-rich domains).

    Unlike many receptors, TNFR1 does not have enzymatic activity. Instead, an oligomeric complex, called the death domain (DD) places the intracellular regions of the receptor into proximity for recruitment of signaling proteins with enzymatic activities to amplify the signal transduction and mediate extrinsic signal induced cell death1.

    TNFα, a ligand of TNFR1 binds to the intracellular death domain of TNFR1 and recruits the TNF receptor-associated DD protein (TRADD), which in turn recruits receptor-interacting protein kinase 1 (RIP1), cellular inhibitor of apoptosis proteins 1 and 2 (cIAP1 and 2), and TNF receptor-associated factor 22.

    TNFR1-mediated signaling provides potent anti-viral activity, however many viruses have evolved to encode and express molecules that specifically inhibit almost every step of TNFR-induced apoptosis and NF-κB signaling pathways3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 16/01/2018

    Anti-Human Tumor Necrosis Factor Receptor 1 (extracellular) (#ANT-041) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. The antibody recognizes an extracellular epitope, and detects the protein in living cells. It has been designed to recognize TNFR1 from human samples only.

    For research purposes only, not for human use