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Anti-HVCN1 Antibody

Hydrogen voltage-gated channel 1, HV1, Voltage sensor domain-only protein, VSOP

Cat #: AHC-001
Alternative Name Hydrogen voltage-gated channel 1, HV1, Voltage sensor domain-only protein, VSOP
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)GDDYHTWNVNYKK, corresponding to amino acid residues 32-44 of mouse HVCN1 (Accession Q3U2S8). Intracellular, N-terminus.
Accession (Uniprot) Number Q3U2S8
Gene ID 74096
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - identical; human - 11/13 amino acid residues identical.
RRID AB_10917155.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.6 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of mouse WEHI B-cell lymphoma (lanes 1 and 4), human HL-60 promyelocytic leukemia (lanes 2 and 5) and human THP-1 acute monocytic leukemia (lanes 3 and 6) cell lysates:
    Western blot analysis of mouse WEHI B-cell lymphoma (lanes 1 and 4), human HL-60 promyelocytic leukemia (lanes 2 and 5) and human THP-1 acute monocytic leukemia (lanes 3 and 6) cell lysates:
    1-3. Anti-HVCN1 Antibody (#AHC-001), (1:200).
    4-6. Anti-HVCN1 Antibody, preincubated with HVCN1 Blocking Peptide (#BLP-HC001).
Indirect flow cytometry
  • Indirect flow cytometry analysis of fixed and permeabilized HL-60 (human promyelocytic leukemia cells) cell line:
    Indirect flow cytometry analysis of fixed and permeabilized HL-60 (human promyelocytic leukemia cells) cell line:
    ___ Unstained cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-HVCN1 Antibody (#AHC-001), (1:25) + goat-anti-rabbit-FITC.
  • The control antigen is not suitable for this application.
References
  1. DeCoursey, T.E. (1991) Biophys. J. 60, 1243.
  2. Ramsey, I.S. et al. (2006) Nature 440, 1213.
  3. Sasaki, M. et al. (2006) Science 312, 589.
  4. Capasso, M. et al. (2011) Trends Cell Biol. 21, 20.
  5. Koch, H.P. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105, 9111.
  6. Lee, S.Y. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105, 7692.
  7. Tombola, F. et al. (2008) Neuron 58, 546.
  8. Demaurex, N. and El Chemaly, A. (2010) J. Physiol. 588.23, 4659.
  9. Musset, B. et al. (2008) Proc. Natl. Acad. Sci. U.S.A.105, 11020.
  10. Schilling, T. et al. (2002) J. Physiol. 545, 93.
Scientific background

Currents measured from voltage-gated proton channels were detected1 long before the channel (HVCN1, also known as HV1 and VSOP) was cloned2,3. HVCN1 has four membrane spanning domains and intracellular N- and C-termini. Interestingly, unlike its voltage-gated ion channel counterparts, HVCN1 has no pore4. Also, functional HVCN1 channels are formed by dimers where each monomer has its own conducting pore, each with its own voltage sensor (voltage sensing occurs similarly to other voltage-gated ion channels)5-8.   

The fundamental role of HVCN1 is to pump out protons, thereby increasing the intracellular pH. The channel is exclusively selective for H+ and opens upon membrane depolarization, although its open state hugely depends on the pH on both sides of the membrane4. Its role is best described in leukocytes where phosphorylation via PKC on a Thr residue potentiates the activity of the channel and increases its open state, thereby increasing the H+ current across the membrane, in this manner mediating optimal NADPH-oxidase (whose optimal activity is at pH 7.5) required for the production of reactive oxygen species (ROS) necessary for phagocytosis to occur4.

Apart from leukocytes, HVCN1 is also expressed in basophils where its activation mediates histamine release4,9. In B cells, it maintains optimal signaling, such that ROS production is maintained high10. HVCN1 was found to regulate human spermatozoa activation. Finally, in the airway mucosa, where it regulates pH, channel gating there, is mostly mediated by differences in pH across the membrane as opposed to the membrane potential4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of mouse HVCN1 channel. Anti-HVCN1 Antibody (#AHC-001) can be used in western blot and indirect flow cytometry applications. It has been designed to recognize HVCN1 from mouse, rat and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Mouse bone marrow dendritic cell lysate (1:200).
    Szteyn, K. et al. (2012) Am. J. Physiol. 303, C204.
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