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Anti-IP3 Receptor-1 (ITPR1) Antibody

Inositol 1,4,5-trisphosphate receptor type 1, IP3R1, InsP3R1
Cat #: ACC-019
Alternative Name Inositol 1,4,5-trisphosphate receptor type 1, IP3R1, InsP3R1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RIGLLGHPPHMNVNPQQPA, corresponding to amino acid residues 2732-2750 of rat IP3R1 (Accession P29994). Intracellular, C-terminus.
Accession (Uniprot) Number P29994
Gene ID 25262
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human, mouse - identical.
RRID AB_2039923.
Purity IgG fraction purified on immobilized Protein A.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.3 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, ip, wb
May also work in: ifc*
Western blot
  • Western blot analysis of rat brain membranes:
    Western blot analysis of rat brain membranes:
    1. Anti-IPReceptor-1 (ITPR1) Antibody (#ACC-019), (1:2000).
    2. Anti-IPReceptor-1 (ITPR1) Antibody, preincubated with IP3 Receptor-1/ITPR1 Blocking Peptide (#BLP-CC019).
Immunoprecipitation
  • Rat superior cervical ganglia (SGC) lysate (Zhang, H. et al. (2013) Neuroscience 254, 70.).
Immunohistochemistry
  • Expression of IP3R1 in mouse cerebellum
    Expression of IP3R1 in mouse cerebellum
    Immunohistochemical staining of mouse cerebellum with Anti-IP3 Receptor-1 (ITPR1) Antibody (#ACC-019). Immunoreactivity appears in Purkinje cells (arrows in A) and their dendritic trees. In addition, axonal processes coursing through cerebellar white matter are visualized (arrow in B).
References
  1. Blondel, O. et al. (1993) J. Biol. Chem. 268, 11356.
  2. V'arnai, P. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 7859.
  3. Cai, W. et al. (2004) J. Biol. Chem. 279, 23691.
Scientific background

Inositol 1,4,5-triphosphate (IP3) serves as a second messenger for many neurotransmitters, hormones and growth factors.1 The binding of IPto its receptor (IP3R), which is a ligand-gated Ca2+ channel, located predominantly at the endoplasmic reticulum, results in a rapid release of Ca2+ from intracellular stores.1

To date, three known isoforms of IP3R are known (designated IP3R1, IP3R2, and IP3R3) that can work as homotetramers or hetrotetramers. All three receptors have six transmembrane domains and a pore region between TM5 and TM6. The N-terminus as well as the C-terminus face the cytoplasm. Each IP3R consists of an N-terminal ligand binding domain (LBD) and a C-terminal domain which is linked by a long regulatory domain. The C-terminus is constitutively active, suggesting that the regulatory domain is required in order to maintain the suppression of channel activity.2

IP3R1 is one of the most important channels responsible for Ca2+ release from intracellular stores and was shown to be the predominant isoform expressed in the central nervous system. IP3R1 is a pivotal player in many neuronal functions, amongst them, neuronal plasticity and neurite extension.3

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title:

Anti-IP3 Receptor-1 (ITPR1) Antibody
Expression of IP3 receptor 1 in TA muscle sections.Immunohistochemical staining of mouse muscle sections using Anti-IP3 Receptor-1 (ITPR1) Antibody (#ACC-019). IP3R1 staining (green) is detected at the neuromuscular junction (upper panel). Staining is almost completely eradicated upon siRNA treatment (middle panel). IP3R1 specific staining is shown upon scrambled RNA (ctRNA) treatment (lower panel).Adapted from Zhu, H. et al. (2011) J. Neurosci. 31, 15269. with permission of the Society for Neuroscience.

Last update: 16/08/2020

Anti-IP3 Receptor-1 (ITPR1) Antibody (#ACC-019) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize IP3R1 from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Rat brain lysate (1:4000).
    Kaya, L. et al. (2013) Biochim. Piophys. Acta 1833, 1421.
  2. Mouse C2C12 myoblast cell lysate. Also tested in siRNA treated cells.
    Zhu, H. et al. (2011) J. Neurosci. 31, 15269.
  3. Rat brain and cerebral artery lysates (1:1000).
    Zhao, G. et al. (2008) Am. J. Physiol. 295, C1376.
  4. Rat A7r5 cell lysate. Also tested in siRNA treated cells.
    Shirane, D. et al. (2004) Nat. Genet. 36, 190.
Immunoprecipitation citations
  1. Rat superior cervical ganglia (SGC) lysate.
    Zhang, H. et al. (2013) Neuroscience 254, 70.
Immunohistochemistry citations
  1. Mouse tiabilis arterior muscle sections.
    Zhu, H. et al. (2011) J. Neurosci. 31, 15269.
  2. Rat brain sections.
    Kaufmann, W.A. et al. (2009) J. Comp. Neurol. 515, 215.
  3. Zebrafish brain sections (1:100).
    Bai, Q. et al. (2007) Nucleic Acids Res. 35, 6501.
Immunocytochemistry citations
  1. Mouse C2C12 myoblast cells.
    Zhu, H. et al. (2011) J. Neurosci. 31, 15269.
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