Anti-IQGAP1 Antibody

IQ Motif Containing GTPase Activating Protein 1, Ras GTPase-Activating-Like Protein IQGAP1, p195
  • New
Cat #: APZ-055
Alternative Name IQ Motif Containing GTPase Activating Protein 1, Ras GTPase-Activating-Like Protein IQGAP1, p195
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)KAYQDRLAYLRSHKDE, corresponding to amino acid residues 793 - 808 of human IQGAP1 (Accession P46940). Intracellular.
    • Anti-IQGAP1 Antibody
    Accession (Uniprot) Number P46940
    Gene ID 8826
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat – 15/16 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ip*
    Western blot
    • Anti-IQGAP1 Antibody
      Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
      1, 2. Anti-IQGAP1 Antibody (#APZ-055), (1:200).
      3, 4. Anti-IQGAP1 Antibody, preincubated with the negative control antigen.
    • Anti-IQGAP1 Antibody
      Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 3) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 2 and 4):
      1, 2. Anti-IQGAP1 Antibody (#APZ-055), (1:200).
      3, 4. Anti-IQGAP1 Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-IQGAP1 Antibody
      Expression of IQGAP1 in mouse cerebellum
      Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-IQGAP1 Antibody (#APZ-055), (1:200), followed by goat anti-rabbit-AlexaFluor-488. IQGAP1 immunoreactivity appears in Purkinje neurons (horizontal arrows) and their dendrites (vertical arrows). Cell nuclei are stained with DAPI (blue).
    References
    1. Abel, A.M. et al. (2015) Mol. Immunol. 65, 336.
    2. Li, Z. et al. (2005) J. Biol. Chem. 280, 13871.
    3. Hedman, A.C. et al. (2015) EMBO Rep. 16, 427.
    4. Gao, C. et al. (2011) J. Neurosci. 31, 8533.
    5. Johnson, M. et al. (2011) Int. J. Biochem. Cell Biol. 43, 65.
    6. Johnson, M. et al. (2009) Cell. Signal. 21, 1471.
    Scientific background

    IQGAP1 is a 190 kDa (1657 amino acid) protein that is ubiquitously expressed and contains six distinct protein-interacting domains with six coiled coil regions1.

    The Ras GTPase-activating-like protein is a scaffold protein involved in regulating various cellular processes; from organizing the actin cytoskeleton, transcription, cell adhesion, and regulating the cell cycle2,3.

    IQGAP1 binds to activated CDC42 but does not stimulate its GTPase activity and it may have a role in cell cycle progression following DNA damage1,2. The protein also regulates NR2A NMDA receptor signaling where it influences cognitive processes4. It also regulates MAPK and Wnt/β-catenin signaling pathways5. It is thought to contribute to tumorigenesis by stimulating cell motility and invasion6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 09/07/2020

    Anti-IQGAP1 Antibody (#APZ-055) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize IQGAP1 from rat, mouse, and human samples.

    For research purposes only, not for human use
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