Anti-KCNK1 (TWIK-1) (extracellular) Antibody

Potassium channel subfamily K member 1, Inward rectifying K+ channel protein TWIK-1, K2P1.1, HOHO1, KCNO1
    Cat #: APC-110
    Alternative Name Potassium channel subfamily K member 1, Inward rectifying K+ channel protein TWIK-1, K2P1.1, HOHO1, KCNO1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide RQELRKLKRRFLEEHEC, corresponding to amino acid residues 53-69 of human KCNK1 (Accession O00180). Extracellular, near the P1 loop.
    • Anti-KCNK1 (TWIK-1) (extracellular) Antibody
    Accession (Uniprot) Number O00180
    Gene ID 3775
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse, pig - identical.
    RRID AB_2040140.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*, lci*
    Western blot
    • Anti-KCNK1 (TWIK-1) (extracellular) Antibody
      Western blot analysis of rat brain lysate:
      1. Anti-KCNK1 (TWIK-1) (extracellular) Antibody (#APC-110), (1:200).
      2. Anti-KCNK1 (TWIK-1) (extracellular) Antibody, preincubated with the control peptide antigen.
    • Anti-KCNK1 (TWIK-1) (extracellular) Antibody
      Western blot analysis of HEK-293-KCNK1 transfected cells:
      1. Anti-KCNK1 (TWIK-1) (extracellular) Antibody (#APC-110), (1:300).
      2. Anti-KCNK1 (TWIK-1) (extracellular) Antibody, preincubated with the control peptide antigen.
    • Anti-KCNK1 (TWIK-1) (extracellular) Antibody
      Expression of KCNK1 in mouse cerebellum
      Immunohistochemical staining of mouse cerebellum using Anti-KCNK1 (TWIK-1) (extracellular) Antibody (#APC-110). A. KCNK1 channel appears in glial processes (red). B. Staining of Purkinje nerve cells with mouse anti-calbindin D28K (a calcium binding protein, green). C. Confocal merge of KCNK1 channel and calbindin D28K demonstrates the separate localization of these proteins.
    1. Lesage, F. et al. (1996) EMBO J. 15, 1004.
    2. Lesage, F. and Lazdunski, M. (2000) Am. J. Physiol. Renal Physiol. 279, F793.
    3. Goldstein, S.A.N. et al. (2003) Nat. Rev. Neurosci. 2, 175.
    Scientific background

    KCNK1 (also named TWIK-1 or K2P1.1) is a member of the 2-pore (2P) domain K+ channels family that, currently, includes 16 members. These channels show little time- or voltage-dependence and are considered to be “leak” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation. The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C termini. 

    K2P channels are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, etc. but are insensitive to the classical Kchannel blockers.

    KCNK1 was the first of the K2P channels to be identified and as its original name indicates (Tandem of P domains in a Weak Inward rectifier K+ channel) the channel behaves as a weak inward rectifier when expressed in heterologous systems. The channel is ubiquitously expressed with the most prominent expression in the brain, kidney and heart.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human K2P1.1 channel. Anti-KCNK1 (TWIK-1) (extracellular) Antibody (#APC-110) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting KCNK1 in living cells. It has been designed to recognize KCNK1 from mouse, rat and human samples.

    For research purposes only, not for human use



    Scientific Background


    Western blot citations
    1. Mouse primary cortical astrocyte lysate (1:500).
      Hwang, E.M. et al. (2014) Nat. Commun. 5, 3227.
    More product citations
    1. Pivonkova, H. et al. (2010) Neurochem. Int. 57, 783.
    2. Zhou, M. et al. (2009) J. Neurosci. 29, 8551.
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