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Anti-KCNK12 (THIK-2) (extracellular) Antibody

Potassium channel subfamily K member 12, Tandem pore domain halothane-inhibited K+ channel 2, K2P12.1
Cat #: APC-169
Alternative Name Potassium channel subfamily K member 12, Tandem pore domain halothane-inhibited K+ channel 2, K2P12.1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)ESPGEAEARARWG, corresponding to amino acid residues 61-73 of rat KCNK12 (Accession Q9ERS1). 1st extracellular loop.
Accession (Uniprot) Number Q9ERS1
Gene ID 64119
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2756753.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat brain synaptosomal fraction (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    Western blot analysis of rat brain synaptosomal fraction (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    1,2. Anti-KCNK12 (THIK-2) (extracellular) Antibody (#APC-169), (1:400).
    3,4. Anti-KCNK12 (THIK-2) (extracellular) Antibody, preincubated with KCNK12/THIK-2 (extracellular) Blocking Peptide (#BLP-PC169).
  • Western blot analysis of mouse lung lysate (lanes 1 and 3) and rat kidney membranes (lanes 2 and 4):
    Western blot analysis of mouse lung lysate (lanes 1 and 3) and rat kidney membranes (lanes 2 and 4):
    1,2. Anti-KCNK12 (THIK-2) (extracellular) Antibody (#APC-169), (1:200).
    3,4. Anti-KCNK12 (THIK-2) (extracellular) Antibody, preincubated with KCNK12/THIK-2 (extracellular) Blocking Peptide (#BLP-PC169).
  • Western blot analysis of human SH-SY5Y neuroblastoma cell lysate:
    Western blot analysis of human SH-SY5Y neuroblastoma cell lysate:
    1. Anti-KCNK12 (THIK-2) (extracellular) Antibody (#APC-169), (1:200).
    2. Anti-KCNK12 (THIK-2) (extracellular) Antibody, preincubated with KCNK12/THIK-2 (extracellular) Blocking Peptide (#BLP-PC169).
Immunohistochemistry
  • Expression of KCNK12 (THIK-2) in rat hippocampus
    Expression of KCNK12 (THIK-2) in rat hippocampus
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-KCNK12 (THIK-2) (extracellular) Antibody (#APC-169), (1:400), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. THIK-2 immunoreactivity (green) in the rat hippocampal CA3 region is detected in neuronal soma (horizontal arrows) and in the dendrite initial segment (vertical arrows). Cell nuclei are stained with DAPI (blue).
References
  1. Kindler, C.H. et al. (2005) Reg. Anesth. Pain Med. 30, 260.
  2. Renigunta, V. et al. (2014) Pflugers. Arch. 466, 1735.
Scientific background

Potassium channels form the largest ion channel family with over 70 genes identified in humans. 2-pore domain channels (K2P) are believed to be responsible for background or leak K+ currents and they are widely expressed in the nervous system. They are involved in resting potential control and firing pattern of excitable cells1. The K2P channel family is comprised of at least 16 members.

The KCNK12 channel (THIK-2, K2P12.1) has two pore domains (M1 and P1) and a long M1-P1 linker. It has a long cytosolic C terminus and a very unusual N-terminal domain containing many cysteine, proline and arginine residues. This N-terminal domain cannot be found in the similar THIK-1 channel and is highly conserved in mammals.

Until recently, the THIK-2 channel was considered to be a “silent” channel and could not be functionally expressed in mammalian and non-mammalian cell lines. When removing the N-terminal both THIK-1 and THIK-2 channels exhibit similar current-voltage relations. In addition, both channels have 64% identical amino-acids in humans and 59% in rats. Only a small fraction of THIK-2 channel is localized to the cell membrane surface and this can be increased by deleting the original retention/retrieval signal RRR in its N-terminus.

The THIK-2 channel is highly expressed in the cortex, cerebellum hippocampus, thalamus, inferior colliculi of the midbrain and the olfactory bulb. It is also expressed in non-neuronal tissues such as the lungs, liver, kidney and spleen2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-KCNK12 (THIK-2) (extracellular) Antibody (#APC-169) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KCNK12 from rat and mouse and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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