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Anti-KCNK16 (TALK-1) (extracellular) Antibody

Potassium two pore domain channel subfamily K member 16, TWIK-related alkaline pH-activated K+ channel 1, K2P16.1

Cat #: APC-170
Alternative Name Potassium two pore domain channel subfamily K member 16, TWIK-related alkaline pH-activated K+ channel 1, K2P16.1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EAWVKGVNPKGNST, corresponding to amino acid residues 75-88 of human KCNK16 (Accession Q96T55). 1st extracellular loop.
Accession (Uniprot) Number Q96T55
Gene ID 83795
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse – identical.
RRID AB_2756754.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*, lci*
Western blot
  • Western blot analysis of rat pancreas lysate:
    Western blot analysis of rat pancreas lysate:
    1. Anti-KCNK16 (TALK-1) (extracellular) Antibody (#APC-170), (1:600).
    2. Anti-KCNK16 (TALK-1) (extracellular) Antibody, preincubated with KCNK16/TALK-1 (extracellular) Blocking Peptide (#BLP-PC170).
  • Western blot analysis of human PANC-1 pancreas ductal adenocarcinoma cell line lysate:
    Western blot analysis of human PANC-1 pancreas ductal adenocarcinoma cell line lysate:
    1. Anti-KCNK16 (TALK-1) (extracellular) Antibody (#APC-170), (1:600).
    2. Anti-KCNK16 (TALK-1) (extracellular) Antibody, preincubated with KCNK16/TALK-1 (extracellular) Blocking Peptide (#BLP-PC170).
References
  1. Dickerson, M.T. et al. (2017) PLoS One 12, e0175069.
  2. Vierra, N.C. et al. (2015) Diabetes 64, 3818.
Scientific background

The KCNK16 gene encodes the TALK-1 channel, an abundant K+ channel transcript found in mouse and in human β-cells. There are four human TALK-1 transcript variants, including two that form functional K+ channels (TALK-1a (transcript variant 2) and TALK1-b (transcript variant 3)1,2. The TALK-1 structure includes 4 transmembrane domains, 2 extracellular loops, and intracellular N- and C-termini. The C-terminal tail of TALK-1 is a critical component that influences the magnitude of TALK-1 channel activation, while the N-terminus is important for protein interaction1,2.

TALK-1, two-pore domain K+ (K2P16.1) channel is responsible for modulating glucose stimulated insulin secretion that relies on β-cell Ca2+ influx. The channel produces outwardly rectifying, non-inactivating K+ currents, which are enhanced by elevations in extracellular pH. TALK-1 is thus pH-sensitive, with increased activity under alkaline conditions. In addition, it can be activated by singlet oxygen and nitric oxide.

A gain-of-function polymorphism in the KCNK16 gene is associated with increased risk for developing type-2 diabetes1,2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/01/2023

Anti-KCNK16 (TALK-1) (extracellular) Antibody (#APC-170) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, analysis. The antibody recognizes an extracellular epitope, and can potentially be used for detecting the protein in living cells. It has been designed to recognize TALK-1 from rat, mouse, and human samples.

For research purposes only, not for human use
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