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Anti-KCNK18 (TRESK) (extracellular) Antibody

Potassium channel subfamily K member 18, TWIK-related spinal cord K+ channel, K2P18.1
Cat #: APC-122
Alternative Name Potassium channel subfamily K member 18, TWIK-related spinal cord K+ channel, K2P18.1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)EAEENPELKKFLDD, corresponding to amino acid residues 62-75 of mouse KCNK18 (Accession Q6VV64). 1st extracellular loop.
Accession (Uniprot) Number Q6VV64
Gene ID 332396
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - 13/14 amino acid residues identical.
RRID AB_2039949.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Unlikely to recognize human samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, lci, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 3) and dorsal root ganglion (lanes 2 and 4) membranes:
    Western blot analysis of rat brain (lanes 1 and 3) and dorsal root ganglion (lanes 2 and 4) membranes:
    1,2. Anti-KCNK18 (TRESK) (extracellular) Antibody (#APC-122), (1:200).
    3,4. Anti-KCNK18 (TRESK) (extracellular) Antibody, preincubated with KCNK18/TRESK (extracellular) Blocking Peptide (#BLP-PC122).
  • Western blot analysis of mouse brain membranes:
    Western blot analysis of mouse brain membranes:
    1. Anti-KCNK18 (TRESK) (extracellular) Antibody (#APC-122), (1:200).
    2. Anti-KCNK18 (TRESK) (extracellular) Antibody, preincubated with KCNK18/TRESK (extracellular) Blocking Peptide (#BLP-PC122).
Immunohistochemistry
  • Expression of KCNK18 in rat dorsal root ganglia
    Expression of KCNK18 in rat dorsal root ganglia
    Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-KCNK18 (TRESK) (extracellular) Antibody (#APC-122), (1:100) followed by Alexa 555-labeled secondary antibody (red staining). Both big/medium sized neurons and small neurons are stained. Note that glial cells and axonal fibers are not stained. Hoechst 33342 is used as the counterstain (blue staining).
Live cell imaging / Immunocytochemistry
  • Expression of KCNK18 in ND7/23 cell line
    Expression of KCNK18 in ND7/23 cell line
    Cell surface detection of KCNK18 in living mouse/rat neuroblastoma x dorsal root ganglion neuron hybrid cell line (ND7/23). A. Extracellular staining of cells with Anti-KCNK18 (TRESK) (extracellular) Antibody (#APC-122), (1:50) followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody (red). Nuclei were visualized with the cell permeable dye Hoechst 33342 (blue staining). B. Show visible light images of the cells shown on (A).
References
  1. Goldstein, S.A.N. et al. (2003) Nat. Rev. Neurosci. 2, 175.
  2. Mathie, A. (2007) J. Physiol. 578, 377.
  3. Liu, C. et al. (2004) Anesth. Analg. 99, 1715.
Scientific background

KCNK18 (also named TWIK-related spinal cord K+ channel, TRESK or K2P18.1) is a member of the 2-pore (2P) domain K+ channel family that currently includes 16 members. These channels show little time- or voltage-dependence and are considered to be “leak” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and control cell excitation.1

The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. It has been proposed that the functional channel unit is a dimer.

Different K2P family members are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, signaling pathways (PKC and PKA), arachidonic acid, etc.

KCNK18 is the only K2P channel, known so far, whose current is activated following Gαq-receptor coupled activation. The enhancement of KCNK18 current involves activation of calcineurin (calcium–calmodulin-dependent phosphatase 2B) following the rise in intracellular calcium that occurs subsequent to Gαq activation.2  In addition, KCNK18 is potently activated by clinical concentrations of volatile anesthetics.3

KCNK18 expression in humans is largely restricted to the spinal cord although in rodents it has a broader expression pattern that includes brain, testis and spleen.

KCNK18 represents the most important background K+ channel in dorsal root ganglion neurons and hence it has been postulated that it has an important role in acute and chronic pain as well as general anesthesia.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse KCNK18. Anti-KCNK18 (TRESK) (extracellular) Antibody (#APC-122) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize TRESK channel from mouse and rat samples. The antibody will unlikely recognize TRESK from human samples.

For research purposes only, not for human use
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