Anti-KCNK5 (TASK-2) Antibody

Potassium channel subfamily K member 5, K2P5.1, TWIK-related acid sensitive K+ channel 2
Cat #: APC-037
Alternative Name Potassium channel subfamily K member 5, K2P5.1, TWIK-related acid sensitive K+ channel 2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)YEQLMNEYNKANSPKGT, amino acid residues 483-499 of human KCNK5 (Accession number O95279). Intracellular, C-terminus.
    Accession (Uniprot) Number O95279
    Gene ID 8645
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 14/17 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.3 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
      • Western blot analysis of rat kidney membranes:
        1. Anti-KCNK5 (TASK-2) Antibody (#APC-037), (1:200).
        2. Anti-KCNK5 (TASK-2) Antibody, preincubated with the control peptide antigen.
        Mouse Ehrlich ascites tumour (EAT) cells (1:250) (Kirkegaard, S.S. et al. (2013) Cell. Physiol. Biochem. 32, 1238.).
      • Expression of KCNK5 in rat pancreas
        Immunohistochemical staining of rat pancreas was visualized using Anti-KCNK5 (TASK-2) Antibody (#APC-037), (1:50). Paraffin embedded sections of rat pancreas showing both endocrine (Islets of Langerhans) and exocrine areas. Strong and highly specific staining is evident only in endocrine cells (arrow). Universal Immuno-alkaline-phosphatase Polymer followed by New Fuchsin Substrate (Histofine, Nichirei Corp.) was used for the color reaction. Hematoxilin is used as the counterstain.
      • KCNK5 (also named TWIK-related acid sensitive K+ channel 2, TASK-2 or K2P5.1) is a member of the 2-pore (2P) domain K+ channels family that includes at least 16 members. These channels show little time- or voltage-dependence and are considered to be “leak” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.

        The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. The functional channel is believed to be a dimer.

        K2P channels are regulated by diverse physical and chemical stimuli including temperature, changes in intracellular pH, mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators.

        As indicated by its name, KCNK5 (K2P5.1) is sensitive to variations in external pH. KCNK5 current is maximal at pH 8.8 but it is only 10% at pH 6.5. 

        The channel is expressed in several epithelial tissues including pancreas, lung, small intestine and specially kidney. In the kidney KCNK5 has an important function in NaHCO3 absorption in the proximal tubule as knockout mice for KCNK5 display metabolic acidosis and hypotension.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/06/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human KCNK5. AntiKCNK5 (TASK-2) Antibody (#APC-037) can be used in western blot and immunohistochemistry applications. It has been designed to recognize KCNK5 from rat, mouse and human samples.

    For research purposes only, not for human use
    Citations
      • Mouse Ehrlich ascites tumour (EAT) cells (1:250).
        Kirkegaard, S.S. et al. (2013) Cell. Physiol. Biochem. 32, 1238.
      • Bando, Y. et al. (2012) Cereb. Cortex doi: 10.1093/cercor/bhs387.
      • Marionneau, C. et al. (2008) J. Mol. Cell. Cardiol. 44, 1002.
      • Gonczi, M. et al. (2006) Br. J. Pharmacol. 147, 496.
      • Cho, S.Y. et al. (2005) J. Physiol. 565.1, 243.
      • Eaton, M.J. et al. (2004) NeuroReport 15, 321.
      • Gardener, M.J. et al. (2004) Br. J. Pharmacol. 142, 192.
      • Lin, W. et al. (2004) J. Neurophysiol. 92, 2909.
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