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Anti-GRIK5 (GluK5) (extracellular) Antibody

Glutamate receptor ionotropic kainate 5, Glutamate receptor KA-2, KA2

Cat #: AGC-042
Alternative Name Glutamate receptor ionotropic kainate 5, Glutamate receptor KA-2, KA2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RRPNFQALSGNER,corresponding to amino acid residues 430-442 of rat kainate receptor GluK5 (Accession Q63273). Extracellular, N-terminus.
Accession (Uniprot) Number Q63273
Gene ID 24407
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human, mouse - identical.
RRID AB_2756600.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat hippocampus lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
    Western blot analysis of rat hippocampus lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
    1,2. Anti-GRIK5 (GluK5) (extracellular) Antibody (#AGC-042), (1:400).
    3,4. Anti-GRIK5 (GluK5) (extracellular) Antibody, preincubated with GRIK5/GluK5 (extracellular) Blocking Peptide (#BLP-GC042).
Immunohistochemistry
  • Expression of kainate receptor GluK5 in mouse hippocampus
    Expression of kainate receptor GluK5 in mouse hippocampus
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-GRIK5 (GluK5) (extracellular) Antibody (#AGC-042), (1:300), followed by goat-anti-rabbit-Alexa-488. GluK5 staining (green) appears in neurons (horizontal arrows) of the pyramidal layer (Pyr) and in apical dendrites (vertical arrows) of mouse hippocampus. Nuclei are stained with DAPI (blue).
References
  1. Weiss, B. et al. (2006) J. Pharmacol. Exp. Ther. 318, 772.
  2. Hollmann, M. and Heinemann, S. (1994) Annu. Rev. Neurosci. 17, 31.
  3. Partin, K.M. et al. (1993) Neuron 11, 1069.
  4. Filla, S.A. et al.(2002) J. Med. Chem. 45, 4383.
  5. Smolders, I. et al. (2002) Nat. Neurosci. 5, 794.
Scientific background

There are three glutamate receptor superfamilies defined by the specific agonist binding to them - NMDA, AMPA and kainate.

Several types of kainate receptors have been cloned and classified. Two of these have a high affinity to kainate (GLUK1 and GLUK2), while GLUK5, as well as other members of the family, have a lower affinity1. GLUK5, GLUK6 and GLUK7 have a 75-80% identical amino acid sequence making them a separate subfamily from the other kainate receptors.

The GLUK5 receptor, similar to its subfamily members, is a ligand-gated ion channel. It has an extracellular N-terminus comprised of eight glycosylation consensus sites, four transmembrane domains arranged in a three-plus-one pattern with a large intracellular domain between units three and four and a C-terminus2. GLUK5 is expressed in DRG neuron cell bodies where currents in these cells are preferentially induced by kainate and not AMPA3 and also in trigeminal neurons where kainate receptor antagonists are effective in a dural plasma protein extravasation model of migraine in rats4.

Little is known about the role of GLUK5 in pathologies. A study examining the role of the receptor in brain pathology using GLUK5 antagonists on hippocampal cells slices, found that blocking of the kainate receptor prevents the development of epileptic activity evoked by muscarinic antagonist. The administration of GLUK5 antagonists to conscious mice in the same study showed that the antagonist prevents limbic seizures induced by hippocampal pilocarpine perfusion without evident side effects. These findings suggest that GLUK5 antagonists may be effective in the treatment of epilepsy and further research in this field is required5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat kainate receptor GluK5. Anti-GRIK5 (GluK5) (extracellular) Antibody (#AGC-042) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize GluK5 from rat, human, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Immunocytochemistry citations
  1. Human peripheral blood mononuclear cells (PBMCs) (1:300).
    Bhandage, A.K. et al. (2017) J. Neuroimmunol. 305, 51.
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