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Anti-KCNMA1 (KCa1.1) (extracellular) Antibody

Large conductance calcium-activated potassium channel subfamily M subunit alpha-1, BKCa alpha, Maxi K+, Slo1
Cat #: APC-151
Alternative Name Large conductance calcium-activated potassium channel subfamily M subunit alpha-1, BKCa alpha, Maxi K+, Slo1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)DSSNPIES(S)QNFYKD, corresponding to amino acid residues 199-213 of rat KCNMA1 (Accession Q62976). 1st extracellular loop.
Accession (Uniprot) Number Q62976
Gene ID 83731
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse, human - 14/15 amino acid residues identical.
RRID AB_10915895.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ifc, ih, wb
May also work in: ic*, ip*, lci*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of rat (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1,2. Anti-KCNMA1 (KCa1.1) (extracellular) Antibody (#APC-151), (1:200).
    3,4. Anti-KCNMA1 (KCa1.1) (extracellular) Antibody, preincubated with KCNMA1/KCa1.1 (extracellular) Blocking Peptide (#BLP-PC151).
Immunohistochemistry
  • Ovine arteriole sections (Tao, X. et al. (2015) Am. J. Physiol. 308, H707.).
Indirect flow cytometry
  • Cell surface detection of KCa1.1 in live intact THP-1 (human acute monocytic leukemia cells) cell line:
    Cell surface detection of KCa1.1 in live intact THP-1 (human acute monocytic leukemia cells) cell line:
    ___ Cells + goat-anti-rabbit-Cy5.
    ___ Cells + Anti-KCNMA1 (KCa1.1) (extracellular) Antibody (#APC-151), (1:20) + goat-anti-rabbit-Cy5.
  • The blocking peptide is not suitable for this application.
References
  1. Nelson, M.T. et al. (1995) Am J. Physiol. Cell Physiol. 268, C799.
  2. Toro, L. et al. (1998) News Physiol. Sci. 13, 112.
  3. Lu, R. et al. (2006) J. Physiol. 570, 65.
Scientific background

The KCa1.1 channel (also known as KCNMA1, BKCa, Maxi K+ or slo) is part of a structurally diverse group of K+ channels that are activated by an increase in intracellular Ca2+. KCa1.1 shows a large single channel conductance when recorded electrophysiologically and hence its name. It differs from the rest of the subfamily members in that it can be activated by both an increase in intracellular Ca2+ and by membrane depolarization. In addition, the KCa1.1 channel structurally differs from the other Ca2+-dependent K+ channels. While the latter group has a topology that resembles that of the voltage-dependent K+ channels, the KCa1.1 channel has an extracellular N-terminus domain as well as an additional transmembrane domain.

KCa1.1 is expressed in virtually all cell types where it causes hyperpolarization and helps to connect intracellular Ca2+ signaling pathways and membrane excitability.

Indeed, KCa1.1 channels play a crucial role in smooth muscle contractility, neuronal spike shaping and neurotransmitter release.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title:

Anti-KCNMA1 (KCa1.1) (extracellular) Antibody
Expression of BKCa (KCNMA1) channel in mouse bladder.Immunohistochemical staining of mouse bladder sections using Anti-KCNMA1 (KCa1.1) (extracellular) Antibody (#APC-151). BK staining (red) is detected in urothelium layer (panels 1, a) and in detrusor layer (panels 1, b). Negative control using secondary antibody only shows insignificant background staining (panel 2). DAPI is used to stain nuclei. Panels a, and b are high magnifications of panel 1.Adapted from Lu, M. et al. (2018) Am. J. Physiol. 314, C643. with permission of the American Physiological Society.

Last update: 16/08/2020

Anti-KCNMA1 (KCa1.1) (extracellular) Antibody (#APC-151) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the channel in living cells. It has been designed to recognize KCNMA1 from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Published figures using this product
  • Expression of KCNMA1 in adult and fetal ovine arteriole myocytes.
    Expression of KCNMA1 in adult and fetal ovine arteriole myocytes.
    Immunohistochemical staining of adult and fetal ovine arteriole myocytes using Anti-KCNMA1 (KCa1.1) (extracellular) Antibody (#APC-151). KCNMA1 staining (green) is detected similarly whether under normal conditions (right panels) or under hypoxia (left panels).
    Adapted from Tao, X. et al. (2015) with permission of the American Physiological Society.
Western blot citations
  1. Human U87-MG glioblastoma cell lysate.
    Rosa, P. et al. (2018) J. Cell. Physiol. 233, 6866.
  2. Rat uterine tissue lysate.
    Novakovic, R. et al. (2013) J. Physiol. Pharmacol. 64, 795.
  3. Rat RPV lysate (1:200).
    Protić, D. et al. (2013) Phytother. Res. 27, 1685.
Immunohistochemistry citations
  1. Mouse bladder sections.
    Lu, M. et al. (2018) Am. J. Physiol. 314, C643.
  2. Human brain sections.
    Rosa, P. et al. (2018) J. Cell. Physiol. 233, 6866.
  3. Mouse kidney sections (1:200).
    Li, Y. et al. (2016) PLoS ONE 11, e0155006.
  4. Human myometrium sections (1:50).
    Novakovic, R. et al. (2015) Mol. Hum. Reprod. 21, 545.
  5. Ovine arteriole sections.
    Tao, X. et al. (2015) Am. J. Physiol. 308, H707.
  6. Human artery tissues (1:50).
    Gojkovic-Bukarica, L. et al. (2011) Eur. J. Pharmacol. 654, 266.
Indirect flow cytometry citations
  1. Human U87-MG glioblastoma cells.
    Rosa, P. et al. (2018) J. Cell. Physiol. 233, 6866.
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