Anti-KCNMA1 (KCa1.1) (extracellular) Antibody

Large conductance calcium-activated potassium channel subfamily M subunit alpha-1, BKCa alpha, Maxi K+, Slo1
    Cat #: APC-151
    Alternative Name Large conductance calcium-activated potassium channel subfamily M subunit alpha-1, BKCa alpha, Maxi K+, Slo1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)DSSNPIES(S)QNFYKD, corresponding to amino acid residues 199-213 of rat KCNMA1 (Accession Q62976). 1st extracellular loop.
        Anti-KCNMA1 (KCa1.1) (extracellular) Antibody
    Accession (Uniprot) Number Q62976
    Gene ID 83731
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse, human - 14/15 amino acid residues identical.
    RRID AB_10915895.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ifc, ih, wb
    May also work in: ic*, ip*, lci*
      • Anti-KCNMA1 (KCa1.1) (extracellular) Antibody
        Western blot analysis of rat (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
        1,2. Anti-KCNMA1 (KCa1.1) (extracellular) Antibody (#APC-151), (1:200).
        3,4. Anti-KCNMA1 (KCa1.1) (extracellular) Antibody, preincubated with the negative control antigen.
      • Ovine arteriole sections (Tao, X. et al. (2015) Am. J. Physiol. 308, H707.).
      • Anti-KCNMA1 (KCa1.1) (extracellular) Antibody
        Cell surface detection of KCa1.1 in live intact THP-1 (human acute monocytic leukemia cells) cell line:
        ___ Cells + goat-anti-rabbit-Cy5.
        ___ Cells + Anti-KCNMA1 (KCa1.1) (extracellular) Antibody (#APC-151), (1:20) + goat-anti-rabbit-Cy5.
        The negative control antigen is not suitable for this application.
    References
    • 1. Nelson, M.T. et al. (1995) Am J. Physiol. Cell Physiol. 268, C799.
    • 2. Toro, L. et al. (1998) News Physiol. Sci. 13, 112.
    • 3. Lu, R. et al. (2006) J. Physiol. 570, 65.
      • The KCa1.1 channel (also known as KCNMA1, BKCa, Maxi K+ or slo) is part of a structurally diverse group of K+ channels that are activated by an increase in intracellular Ca2+. KCa1.1 shows a large single channel conductance when recorded electrophysiologically and hence its name. It differs from the rest of the subfamily members in that it can be activated by both an increase in intracellular Ca2+ and by membrane depolarization. In addition, the KCa1.1 channel structurally differs from the other Ca2+-dependent K+ channels. While the latter group has a topology that resembles that of the voltage-dependent K+ channels, the KCa1.1 channel has an extracellular N-terminus domain as well as an additional transmembrane domain.

        KCa1.1 is expressed in virtually all cell types where it causes hyperpolarization and helps to connect intracellular Ca2+ signaling pathways and membrane excitability.

        Indeed, KCa1.1 channels play a crucial role in smooth muscle contractility, neuronal spike shaping and neurotransmitter release.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
    IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-KCNMA1 (KCa1.1) (extracellular) Antibody
    Expression of BKCa (KCNMA1) channel in mouse bladder.Immunohistochemical staining of mouse bladder sections using Anti-KCNMA1 (KCa1.1) (extracellular) Antibody (#APC-151). BK staining (red) is detected in urothelium layer (panels 1, a) and in detrusor layer (panels 1, b). Negative control using secondary antibody only shows insignificant background staining (panel 2). DAPI is used to stain nuclei. Panels a, and b are high magnifications of panel 1.Adapted from Lu, M. et al. (2018) Am. J. Physiol. 314, C643. with permission of the American Physiological Society.

    Last update: 10/11/2019

    Anti-KCNMA1 (KCa1.1) (extracellular) Antibody (#APC-151) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the channel in living cells. It has been designed to recognize KCNMA1 from human, rat, and mouse samples.

    For research purposes only, not for human use
    Citations
      • Anti-KCNMA1 (KCa1.1) (extracellular) Antibody
        Expression of KCNMA1 in adult and fetal ovine arteriole myocytes.
        Immunohistochemical staining of adult and fetal ovine arteriole myocytes using Anti-KCNMA1 (KCa1.1) (extracellular) Antibody (#APC-151). KCNMA1 staining (green) is detected similarly whether under normal conditions (right panels) or under hypoxia (left panels).
        Adapted from Tao, X. et al. (2015) with permission of the American Physiological Society.
      • Rat uterine tissue lysate.
        Novakovic, R. et al. (2013) J. Physiol. Pharmacol. 64, 795.
      • Rat RPV lysate (1:200).
        Protić, D. et al. (2013) Phytother. Res. 27, 1685.
      • Mouse bladder sections.
        Lu, M. et al. (2018) Am. J. Physiol. 314, C643.
      • Mouse kidney sections (1:200).
        Li, Y. et al. (2016) PLoS ONE 11, e0155006.
      • Human myometrium sections (1:50).
        Novakovic, R. et al. (2015) Mol. Hum. Reprod. 21, 545.
      • Ovine arteriole sections.
        Tao, X. et al. (2015) Am. J. Physiol. 308, H707.
      • Human artery tissues (1:50).
        Gojkovic-Bukarica, L. et al. (2011) Eur. J. Pharmacol. 654, 266.
    Related Products
        • Guinea pig Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody (#AGP-014) is raised in guinea pig and can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit. This antibody has been raised against the same epitope as #APC-021.