Anti-KCNT1 (Slack) Antibody

KCa4.1, KNa1.1, Slo2.2 Na+-activated K+ channel, Potassium channel subfamily T member 1
    Cat #: APC-124
    Alternative Name KCa4.1, KNa1.1, Slo2.2 Na+-activated K+ channel, Potassium channel subfamily T member 1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    Immunogen
    • Peptide (C)KQEEKQNRRGLAG, corresponding to amino acid residues 619-631 of rat Slack (Accession Q9Z258). Intracellular, C-terminal.
    • Anti-KCNT1 (Slack) Antibody
    Accession (Uniprot) Number Q9Z258
    Gene ID 60444
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 12/13 amino acid residues identical.
    RRID AB_10557314.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity Unlikely to recognize human samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
    Western blot
    • Anti-KCNT1 (Slack) Antibody
      Western blot analysis of rat brain membrane:
      1. Anti-KCNT1 (Slack) Antibody (#APC-124), (1:200).
      2. Anti-KCNT1 (Slack) Antibody, preincubated with the control peptide antigen.
    • Anti-KCNT1 (Slack) Antibody
      Western blot analysis of mouse brain membrane:
      1. Anti-KCNT1 (Slack) Antibody (#APC-124), (1:200).
      2. Anti-KCNT1 (Slack) Antibody, preincubated with the control peptide antigen.
    References
    1. Bhattacharjeea, A and Kaczmarek, L.K. (2005) Trends Neurosci. 28, 422.
    2. Chen, H. et al. (2009) J. Neurosci. 29, 5654.
    3. Tamsett, T. J. et al. (2009) J. Neurosci. 29, 5127.
    Scientific background

    KCa4.1 (Na+-activated K+ channel, Slack, Slo2.2, KCNT1) is a member of a subgroup within the Ca2+ activated K+ channel family. The Slack (Sequence like a Ca2+-activated K+ channel) and Slick (Sequence like an intermediate conductance K+ channel, KCNT2) genes, which encode Na+-activated K+ (KNa) channels, are expressed at high levels in neurons in several areas of the nervous system1.

    Although KCa4.1 is functionally a Na+-activated K+ channel, it is termed KCa by the IUPHAR nomenclature, due to its sequence homology to other KCa channels.

    The family’s protein topology consists of six transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels, the functional unit for KCa4.1 channels is composed of four subunits that can assemble as either homo- or heteromers with KCNT2 (Slick). KCa4.1 and Slick subunits coassemble to form heteromeric channels that differ from the homomers in their unitary conductance, kinetic behavior, subcellular localization, and response to activation of protein kinase C. Heteromer formation requires the N-terminal domain of Slack-B, one of the alternative splice variants of the KCa4.1 channel. This cytoplasmic N-terminal domain of Slack-B also facilitates the localization of heteromeric KNa channels to the plasma membrane2.

    K+ channels sensitive to intracellular Na+, termed KNa channels, shape neuronal excitability. KNa channels contribute to the adaptation of firing rates and to slow after-hyperpolarizations that follow repetitive firing. In certain neurons, they also appear to be activated by Na+ influx accompanying single action potentials. Their molecular properties also suggest that these channels contribute to the response of neurons to hypoxia1. KCa4.1 channels were shown to be expressed also in dorsal root ganglion (DRG) neurons, where these channels were found to contain a NAD+ binding sequence and to be modulated by this endogenous compound3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the intracellular C-terminal domain of rat KCNT1. Anti-KCNT1 (Slack) Antibody (#APC-124) can be used in western blot applications, and was designed to recognize KCNT1 from rat and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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