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Anti-KChIP1 (KCNIP1) Antibody

KV channel-interacting protein 1, VABP, Vesicle APC-binding protein, A-type potassium channel modulatory protein 1, Potassium channel-interacting protein 1

Cat #: APC-141
Alternative Name KV channel-interacting protein 1, VABP, Vesicle APC-binding protein, A-type potassium channel modulatory protein 1, Potassium channel-interacting protein 1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)SLQTKQRRPSKD, corresponding to amino acid residues 10-21 of rat KChIP1 (Accession Q8R426). N-terminal part.
Accession (Uniprot) Number Q8R426
Gene ID 65023
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_10917754.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat brain lysate:
    Western blot analysis of rat brain lysate:
    1. Anti-KChIP1 (KCNIP1) Antibody (#APC-141), (1:200).
    2. Anti-KChIP1 (KCNIP1) Antibody, preincubated with KChIP1/KCNIP1 Blocking Peptide (#BLP-PC141).
References
  1. Wang, K. (2008) Neurochem. Res. 33, 1964.
  2. Shibata, R. et al. (2003) J. Biol. Chem. 278, 36445.
  3. An, W.F. et al. (2000) Nature 403, 553.
  4. Holmqvist, M.H. et al. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, 1035.
  5. Xia, K. et al. (2010) Molec. Brain 3, 1.
  6. Xiong, H. et al. (2004) Brain Res. Mol. Brain Res. 128, 103.
Scientific background

Voltage-gated K+ (KV) channels form functional entities by the assembly of four α subunits and auxiliary subunits. Various auxiliary subunits are known to interact with KV channels thereby modulating various properties such as gating, activation and inactivation of the channels as well as influencing trafficking of the channels to the cell’s plasma membrane1.

KChIPs (KV channel interacting proteins) are cytoplasmic proteins which belong to the neural Ca2+ sensor (NCS) family of Ca2+ binding EF-hand proteins. To date, KChIP1-4 have been identified. All four KChIPs have a conserved C-terminal domain, which has four EF-hand-like Ca2+ binding motifs. The N-terminal region differs among the various KChIPs and attributes different properties regarding the regulation of KV channels1. KChIPs regulate different properties of KV channels such as their cell surface expression (mediated by proper trafficking of the various subunits), channel assembly and gating1-4. Specifically, KChIP1 strongly regulates the activity of the KV4 channel family1. KChIP1 has also been found to assist GABA-mediated IPSCs (inhibitory postsynaptic currents) by increasing the release of presynaptic transmitter5. Furthermore, KChIP knockout mice display an increase in anxiety-like behavior compared to their wild type counterparts5.

KChIP1, 3, and 4 are mostly expressed in the brain while KChIP2 is expressed in the heart as well as in the brain4-6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KChIP1. Anti-KChIP1 (KCNIP1) Antibody (#APC-141) can be used for western blot analysis. It has been designed to recognize KChIP1 from human mouse and rat samples.

For research purposes only, not for human use
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