Anti-KChIP3 (CSEN, DREAM) Antibody

Calsenilin, KCNIP3, KV channel-interacting protein 3, A-type potassium channel modulatory protein 3, DRE-antagonist modulator
    Cat #: APC-143
    Alternative Name Calsenilin, KCNIP3, KV channel-interacting protein 3, A-type potassium channel modulatory protein 3, DRE-antagonist modulator
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)SKREGIKWQRPR, corresponding to amino acid residues 18-31 of rat KChIP3 (Accession Q9JM47). Cytoplasmic, N-terminus.
    • Anti-KChIP3 (CSEN, DREAM) Antibody
    Accession (Uniprot) Number Q9JM47
    Gene ID 65199
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human, mouse - 11/12 amino acid residues identical.
    RRID AB_2341037.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
    Western blot
    • Anti-KChIP3 (CSEN, DREAM) Antibody
      Western blot analysis of human T-cell lymphoma Jurkat cell lysates (lanes 1 and 4), mouse heart lysates (lanes 2 and 5) and rat brain lysates (lanes 3 and 6):
      1-3. Anti-KChIP3 (CSEN, DREAM) Antibody (#APC-143), (1:200).
      4-6. Anti-KChIP3 (CSEN, DREAM) Antibody, preincubated with the negative control antigen.
    References
    1. Wang, K. (2008) Neurochem. Res. 33, 1964.
    2. Shibata, R. et al. (2003) J. Biol. Chem. 278, 36445.
    3. An, W.F. et al. (2000) Nature 403, 553.
    4. Holmqvist, M.H. et al. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, 1035.
    5. Norris, A.J. et al. (2010) J. Neurosci. 30, 13644.
    6. Xia, K. et al. (2010) Molec. Brain 3, 1.
    7. Xiong, H. et al. (2004) Brain Res. Mol. Brain Res. 128, 103.
    Scientific background

    Voltage-gated K+ (KV) channels form functional entities by the assembly of four α subunits and auxiliary subunits. Various auxiliary subunits are known to interact with KV channels thereby modulating various properties such as gating, activation and inactivation of the channels as well as influencing trafficking of the channels to the cell’s plasma membrane1.

    KChIPs (KV channel interacting proteins) are cytoplasmic proteins which belong to the neural Ca2+ sensor (NCS) family of Ca2+ binding EF-hand proteins. To date, KChIP1-4 have been identified. All four KChIPs have a conserved C-terminal domain, which has four EF-hand-like Ca2+ binding motifs. The N-terminal region differs among the various KChIPs and attributes different properties regarding the regulation of KV channels1. KChIPs regulate different properties of KV channels such as their cell surface expression (mediated by proper trafficking of the various subunits), channel assembly and gating1-4. Specifically, KChIP2, 3 and 4 strongly regulate the activity of the KV4 channel family in cortical pyramidal neurons5. Furthermore, KChIP knockout mice display an increase in anxiety-like behavior compared to their wild type counterparts6.

    KChIP1, 3, and 4 are mostly expressed in the brain while KChIP2 is expressed in the heart as well as in the brain4-7.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Calsenilin. Anti-KChIP3 (CSEN, DREAM) Antibody (#APC-143) can be used for western blot analysis. It has been designed to recognize Calsenilin from human mouse and rat samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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