- Peptide (C)SKREGIKWQRPR, corresponding to amino acid residues 18-31 of rat KChIP3 (Accession Q9JM47). Cytoplasmic, N-terminus.
- Western blot analysis of human T-cell lymphoma Jurkat cell lysates (lanes 1 and 4), mouse heart lysates (lanes 2 and 5) and rat brain lysates (lanes 3 and 6):1-3. Anti-KChIP3 (CSEN, DREAM) Antibody (#APC-143), (1:200).
4-6. Anti-KChIP3 (CSEN, DREAM) Antibody, preincubated with KChIP3/CSEN Blocking Peptide (#BLP-PC143).
- Wang, K. (2008) Neurochem. Res. 33, 1964.
- Shibata, R. et al. (2003) J. Biol. Chem. 278, 36445.
- An, W.F. et al. (2000) Nature 403, 553.
- Holmqvist, M.H. et al. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, 1035.
- Norris, A.J. et al. (2010) J. Neurosci. 30, 13644.
- Xiong, H. et al. (2004) Brain Res. Mol. Brain Res. 128, 103.
Voltage-gated K+ (KV) channels form functional entities by the assembly of four α subunits and auxiliary subunits. Various auxiliary subunits are known to interact with KV channels thereby modulating various properties such as gating, activation and inactivation of the channels as well as influencing trafficking of the channels to the cell’s plasma membrane1.
KChIPs (KV channel interacting proteins) are cytoplasmic proteins which belong to the neural Ca2+ sensor (NCS) family of Ca2+ binding EF-hand proteins. To date, KChIP1-4 have been identified. All four KChIPs have a conserved C-terminal domain, which has four EF-hand-like Ca2+ binding motifs. The N-terminal region differs among the various KChIPs and attributes different properties regarding the regulation of KV channels1. KChIPs regulate different properties of KV channels such as their cell surface expression (mediated by proper trafficking of the various subunits), channel assembly and gating1-4. Specifically, KChIP2, 3 and 4 strongly regulate the activity of the KV4 channel family in cortical pyramidal neurons5. Furthermore, KChIP knockout mice display an increase in anxiety-like behavior compared to their wild type counterparts6.
KChIP1, 3, and 4 are mostly expressed in the brain while KChIP2 is expressed in the heart as well as in the brain4-6.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Calsenilin. Anti-KChIP3 (CSEN, DREAM) Antibody (#APC-143) can be used for western blot analysis. It has been designed to recognize Calsenilin from human mouse and rat samples.