Anti-KCNE3 (MiRP2) Antibody

Potassium channel subunit β MiRP2, MinK-related peptide 2, Potassium voltage-gated channel subfamily E member 3, HOKPP
    Cat #: APC-118
    Alternative Name Potassium channel subunit β MiRP2, MinK-related peptide 2, Potassium voltage-gated channel subfamily E member 3, HOKPP
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)RSRKVDKRSDPYH, corresponding to amino acid residues 81-93 of human KCNE3 (Accession Q9Y6H6). Intracellular, C-terminal part.
        Anti-KCNE3 (MiRP2) Antibody
    Accession (Uniprot) Number Q9Y6H6
    Gene ID 10008
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse, dog, pig -  identical.
    RRID AB_2039929.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
      • Anti-KCNE3 (MiRP2) Antibody
        Western blot analysis of rat kidney (lanes 1 and 3) and heart (lanes 2 and 4) membranes:
        1,2. Anti-KCNE3 (MiRP2) Antibody (#APC-118), (1:200).
        3,4. Anti-KCNE3 (MiRP2) Antibody, preincubated with the negative control antigen.
      • Rat kidney sections.

        Mouse vomeronasal organ (VNO) (1:250) (Hagendorf, S. et al. (2009) J. Neurosci29, 206.).
      • Human colon carcinoma cell line HT29cl.19A (Rapetti-Mauss, M. et al. (2013) J. Physiol. 591, 2813.).
    References
      • KCNE3 (or MiRP2) is a member of a family of proteins that regulate the activity of voltage-dependent K+ channels. Other members of the family are KCNE1 (IsK, MiNK), KCNE2 (MiRP1), KCNE4 (MiRP3) and KCNE5 (MiRP4).

        KCNE1 is the founding member of the family and was initially believed to form a K+ channel itself, but was later recognized to work as a regulatory b subunit associated with the KV7.1 (KCNQ1) a protein. KCNE3 was discovered based on its homology with KCNE1.

        The KCNE regulatory subunits are small proteins (14-20 kD) with a type-1 integral membrane topology. It is believed that both the cytoplasmic C-terminus tail and the transmembrane domain are necessary for the interaction with the a subunits. The stoichiometry of the KCNE subunits with their partner a subunits in the native channels is not clear and ratios ranging from 2 to 14 KCNE subunits per 4 a subunits have been proposed.

        KCNE3 is relatively widely expressed in several tissues with prominent expression in the kidney and skeletal muscle.

        KCNE3 is quite promiscuous and associations with KV7.1, KV3.4, KV7.4 (KCNQ4), KV11.1 (HERG), KV2.1 and KV3.1b have been demonstrated. The best characterized interactions are with the former two proteins. KCNE3 interacts with KV7.1 in epithelial cells of the gastrointestinal tract where it appears to be important for Na+ absorption. In skeletal muscle, KCNE3 couples to KV3.4 to regulate muscle function. Indeed, a mutation in KCNE3 (R83H) has been associated with an inherited form of periodic paralysis (Thyrotoxic hypokalemic periodic paralysis).

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-KCNE3 (MiRP2) AntibodyKnockout validation of Anti-KCNE3 (MiRP2) Antibody in mouse spiral ganglion neurons.Immunocytochemical staining of mouse spiral ganglion neurons (SGNs). Wild type cells and Kcne3-/- knockout cells were stained with Anti-KCNE3 (MiRP2) Antibody (#APC-118). KCNE3 was detected in the SGNs from wild type (upper panels) but not in Kcne3-/- cells (lower panels).Adapted from Wang, W. et al. (2014) J. Biol. Chem. 289, 16802. with permission of The American Society for Biochemistry and Molecular Biology.

    Last update: 24/01/2020

    Anti-KCNE3 (MiRP2) Antibody (#APC-118) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize KCNE3 from rat, mouse, and human samples.

    For research purposes only, not for human use
    Citations
      • Western blot analysis of mouse hind limb lysate. Tested in KCNE3-/- mice.
        King, E.C. et al. (2017) FASEB J. 31, 2937.
      • Immunocytochemical staining of mouse SGN cells. Tested in Kcne3-/- knockout cells.
        Wang, W. et al. (2014) J. Biol. Chem. 289, 16802.
      • Mouse hind limb lysate. Also tested in KCNE3-/- mice.
        King, E.C. et al. (2017) FASEB J. 31, 2937.
      • Rat rectal colon sections.
        Inagaki, A. et al. (2019) Pflugers Arch. 471, 313.
      • Mouse SGN sections.
        Wang, W. et al. (2014) J. Biol. Chem. 289, 16802.
      • Mouse vomeronasal organ (VNO) (1:250).
        Hagendorf, S. et al. (2009) J. Neurosci. 29, 206.
      • Mouse SGN cells. Also tested in Kcne3-/- knockout cells.
        Wang, W. et al. (2014) J. Biol. Chem. 289, 16802.
      • Human colon carcinoma cell line HT29cl.19A.
        Rapetti-Mauss, M. et al. (2013) J. Physiol. 591, 2813.
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