Anti-Kir2.2 (KCNJ12) Antibody

Inward rectifier K+ channel Kir2.2, IRK-2, IRK2, ATP-sensitive inward rectifier potassium channel 12
    Cat #: APC-042
    Alternative Name Inward rectifier K+ channel Kir2.2, IRK-2, IRK2, ATP-sensitive inward rectifier potassium channel 12
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)EVATDRDGRSPQPEHDFDR, corresponding to amino acid residues 391-409 of rat Kir2.2 (Accession P52188). Intracellular, C-terminal part.
    Accession (Uniprot) Number P52188
    Gene ID 117052
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse identical or 16/19 amino acid residues identical; human 13/19 amino acid residues identical.
    RRID AB_2040109.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.39 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, wb
    May also work in: ifc*, ih*, ip*
      • Anti-Kir2.2 (KCNJ12) Antibody
        Western blot analysis of rat kidney (lanes 1 and 3) and brain (lanes 2 and 4) membranes:
        1,2. Anti-Kir2.2 (KCNJ12) Antibody (#APC-042), (1:200).   
        3,4. Anti-Kir2.2 (KCNJ12) Antibody, preincubated with the control peptide antigen.
        Human retinal pigment epithelium (1:300) (Yang, D. et al. (2008) Exp. Eye Res. 87, 176.).
      • Neonatal rat cardiomyocytes (NRCMs) (1:200), (He, Y. et al. (2008) FEBS Lett. 582, 2338.).
    References
      • Kir2.2 is a member of the inward rectifying K+ channel family. The Kir2 subfamily comprises 4 known members: Kir2.1, Kir2.2, Kir2.3 and Kir2.4. The Kir2 subfamily members are strong inward rectifiers and are believed to be involved in maintaining the resting membrane potential and buffering of extracellular K+.

        The Kir2.2 subunit is expressed in the CNS, kidney and heart where it has been found to form heteromeric channels with Kir2.1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
    IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 08/11/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat IRK-2 channel. Anti-Kir2.2 (KCNJ12) Antibody (#APC-042) can be used in western blot, and immunocytochemistry applications. It has been designed to recognize IRK-2 from human, rat and mouse samples.

    For research purposes only, not for human use
    Citations
      • Human THP-1 cells.
        Kim, K.S. et al. (2015) J. Immunol. 195, 3345.
      • Canine heart lysate.
        Bonilla, I.M. et al. (2012) J. Appl. Physiol. 113, 1772.
      • Human retinal pigment epithelial (1:300).
        Yang, D. et al. (2008) Exp. Eye Res. 87, 176.
      • Human THP-1 cells (1:200).
        Kim, K.S. et al. (2015) J. Immunol. 195, 3345.
      • Neonatal rat cardiomyocytes (NRCMs) (1:200).
        He, Y. et al. (2008) FEBS Lett. 582, 2338.
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