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Anti-KCNJ15 (Kir4.2) Antibody

ATP-sensitive inward rectifier potassium channel 15, Potassium channel inwardly rectifying subfamily J member 15, Kir1.3, IRKK

Cat #: APC-058
Alternative Name ATP-sensitive inward rectifier potassium channel 15, Potassium channel inwardly rectifying subfamily J member 15, Kir1.3, IRKK
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EKQKLEEQYRQEDQRERELR, corresponding to amino acid residues 347-366 of mouse KCNJ15 (Accession O88932). Intracellular, C-terminal part.
Accession (Uniprot) Number O88932
Gene ID 16516
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Guinea pig - 19/20 amino acid residues identical; human - 18/20 amino acid residues identical.
RRID AB_2040122.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.6 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat kidney membranes:
    Western blot analysis of rat kidney membranes:
    1. Anti-KCNJ15 (Kir4.2) Antibody (#APC-058), (1:200).
    2. Anti-KCNJ15 (Kir4.2) Antibody, preincubated with KCNJ15/Kir4.2 Blocking Peptide (#BLP-PC058).
  • Human retina lysate (Yang, D. et al. (2008) Exp. Eye Res. 87, 176.).
Immunohistochemistry
  • Rat retina sections (1:100) (Tian, M. et al. (2003) Neurosci. Lett. 345, 9.).
References
  1. Hill, C.E. et al. (2002) Am. J. Physiol. 282, G233.
  2. Pessia, M. et al. (2001) J. Physiol. 532, 359.
Scientific background

Kir4.2 is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.

The family’s topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunit that can assembly as either homo or heteromers.

Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.

Kir4.2 is a member of the Kir4 subfamily that includes one other member: Kir4.1. Kir4.2 can co-assemble with Kir4.1 but also with other Kir channels such as Kir1.1 and Kir5.1.2

The Kir4 subfamily has been classified as weak rectifiers with intermediate conductance. 

Kir4.2 is expressed in liver, kidney, pancreas lung and testis. Its physiological function is not well understood but it has been suggested that it could be involved in the regulation of K+ efflux in epithelial cells such as hepatocytes or lung cells.1

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/06/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the mouse Kir4.2 channel. Anti-KCNJ15 (Kir4.2) Antibody (#APC-058) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Kir4.2 from rat, mouse and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Human retina lysate.
    Yang, D. et al. (2008) Exp. Eye Res. 87, 176.
Immunohistochemistry citations
  1. Rat retina sections (1:100).
    Tian, M. et al. (2003) Neurosci. Lett. 345, 9.
More product citations
  1. Prüss, H. et al. (2011) Neuroscience 180, 19.
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