- Peptide (C)EKQKLEEQYRQEDQRERELR, corresponding to amino acid residues 347-366 of mouse KCNJ15 (Accession O88932). Intracellular, C-terminal part.
- Western blot analysis of rat kidney membranes:1. Anti-KCNJ15 (Kir4.2) Antibody (#APC-058), (1:200).
2. Anti-KCNJ15 (Kir4.2) Antibody, preincubated with KCNJ15/Kir4.2 Blocking Peptide (#BLP-PC058).
- Human retina lysate (Yang, D. et al. (2008) Exp. Eye Res. 87, 176.).
- Rat retina sections (1:100) (Tian, M. et al. (2003) Neurosci. Lett. 345, 9.).
Kir4.2 is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.
The family’s topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunit that can assembly as either homo or heteromers.
Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.
The Kir4 subfamily has been classified as weak rectifiers with intermediate conductance.
Kir4.2 is expressed in liver, kidney, pancreas lung and testis. Its physiological function is not well understood but it has been suggested that it could be involved in the regulation of K+ efflux in epithelial cells such as hepatocytes or lung cells.1
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the mouse Kir4.2 channel. Anti-KCNJ15 (Kir4.2) Antibody (#APC-058) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Kir4.2 from rat, mouse and human samples.