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Anti-Kir6.1 (KCNJ8) Antibody

ATP-sensitive inward rectifier potassium channel 8, uKATP-1

Cat #: APC-105
Alternative Name ATP-sensitive inward rectifier potassium channel 8, uKATP-1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KRNSMRRNNSMRRSN, corresponding to amino acid residues 382-396 of rat Kir6.1 (Accession Q63664). Intracellular, C-terminal part.
Accession (Uniprot) Number Q63664
Gene ID 25472
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human, pig, rabbit - 15/16 amino acid residues identical.
RRID AB_2039945.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity The antibody is specific for Kir6.1 and does not cross react with Kir6.2.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, ip, wb
May also work in: ic*, ifc*
Western blot
  • Western blot analysis of rat heart membranes:
    Western blot analysis of rat heart membranes:
    1. Anti-Kir6.1 (KCNJ8) Antibody (#APC-105), (1:200).
    2. Anti-Kir6.1 (KCNJ8) Antibody, preincubated with Kir6.1/KCNJ8 Blocking Peptide (#BLP-PC105).
  • Western blot analysis of rat cortex lysate:
    Western blot analysis of rat cortex lysate:
    1. Anti-Kir6.1 (KCNJ8) Antibody (#APC-105), (1:200).
    2. Anti-Kir6.1 (KCNJ8) Antibody, preincubated with Kir6.1/KCNJ8 Blocking Peptide (#BLP-PC105).
  • Human umbilical vein segment lysate (Protić, D. et al. (2014) Phytother. Res. 28, 1412.).
Immunoprecipitation
  • Mouse heart lysate (Li, J. et al. (2010) J. Biol. Chem. 285, 28723.).
Immunohistochemistry
  • Expression of Kir6.1 in rat uterus
    Expression of Kir6.1 in rat uterus
    Immunohistochemical stain of pregnant rat longitudinal section of the myometrium using Anti-Kir6.1 (KCNJ8) Antibody (#APC-105), (1:50).  Strong and specific staining is evident in smooth muscles cells both in the myometrium (blue arrow) and muscular layers of blood vessels (green arrow). Peroxidase reaction with DAB were used for the color reaction. Hematoxilin is used as the counterstain.
  • Mouse brain sections (1:100) (Parsons, M.P. and Hirasawa, M. (2010) J. Neurosci. 30, 8061.).
References
  1. Baukrowitz, T. and Fakler, B. (2000) Eur. J. Biochem. 267, 5842.
  2. Aguilar-Bryan, L. et al. (1998) Physiol. Rev. 78, 227.
Scientific background

Kir6.1 is a member of the inward rectifier K+ channels (Kir channels), a large family of voltage-independent K+ channels largely involved in stabilization of the membrane resting potential and in K+ transport across membranes.

Kir6.1, like its close relative Kir6.2, is highly sensitive to inhibition by intracellular ATP. Closure of the channel leads to membrane depolarization thereby coupling intracellular metabolism to cellular excitability.

Kir6.1 presents the common topology of the inward-rectifier superfamily: two transmembrane domains flanking a highly conserved pore region with the N- and C-termini located intracellularly.

The functional ATP sensitive channel (KATP) is composed of octamers of four Kir6.x subunits and four members of the sulfonylurea receptor family SUR1, SUR2A and SUR2B.

The channel's tissue distribution is relatively broad with expression detected in heart, brain, and smooth muscle.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title:

Anti-Kir6.1 (KCNJ8) AntibodyExpression of Kir6.1 in mouse brain sections.Immunohistochemical staining of mouse brain sections using Anti-Kir6.1 (KCNJ8) Antibody (#APC-105). Kir6.1 staining (green) is detected and coincides with orexin neurons.Adapted from Parsons, M.P. and Hirasawa, M. (2010) J. Neurosci. 30, 8061. with permission of the Society for Neuroscience.

Last update: 11/04/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Kir6.1. Anti-Kir6.1 (KCNJ8) Antibody (#APC-105) can be used in western blot, immunoprecipitation and immunohistochemistry applications. It has been designed to recognize Kir6.1 from human, rat and mouse samples. The antibody is specific for Kir6.1 and does not cross react with Kir6.2.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Human myometrium tissue lysate.
    Novakovic, R. et al. (2015) Mol. Hum. Reprod. 21, 545.
  2. Human umbilical vein segment lysate.
    Protić, D. et al. (2014) Phytother. Res. 28, 1412.
  3. Rat RPV lysate.
    Protić, D. et al. (2014) Phytother. Res. 28, 1412.
  4. Mouse heart mitochindria lysate.
    Fancher, I.S. et al. (2013) Life Sci. 92, 664.
  5. Rat uterine tissue lysate.
    Novakovic, R. et al. (2013) J. Physiol. Pharmacol. 64, 795.
  6. Rat heart lysate (1:200).
    Yang, Z.W. et al. (2011) Acta Pharmacol. Sin. 32, 194.
  7. Rat trigeminal ganglia lysate (1:500).
    Niu, K. et al. (2011) Neuroscience 180, 344.
  8. Mouse heart lysate.
    Li, J. et al. (2010) J. Biol. Chem. 285, 28723.
  9. Rat brain lysate (1:200).
    Zoga, V. et al. (2010) Mol. Pain 6, 1.
  10. Rat vascular smooth muscle cell lysate (1:300).
    Fan, L.H. et al. (2009) Nutrition 25, 359.
  11. Rat vascular smooth muscle cell lysate (1:300).
    Fan, L.H. et al. (2009) Biomed. Pharmacother. 63, 165.
Immunoprecipitation citations
  1. Mouse heart lysate.
    Li, J. et al. (2010) J. Biol. Chem. 285, 28723.
Immunohistochemistry citations
  1. Human myometrium sections (1:50).
    Novakovic, R. et al. (2015) Mol. Hum. Reprod. 21, 545.
  2. Human artery tissues (1:50).
    Gojkovic-Bukarica, L. et al. (2011) Eur. J. Pharmacol. 654, 266.
  3. Human HSV and HIMA tissues (1:50).
    Gojkovic-Bukarica, L.C. et al. (2011) J. Cardiovasc. Pharmacol. 57, 648.
  4. Rat trigeminal ganglia sections (1:1000).
    Niu, K. et al. (2011) Neuroscience 180, 344.
  5. Rat heart sections (1:100).
    Yang, Z.W. et al. (2011) Acta Pharmacol. Sin. 32, 194.
  6. Mouse brain sections (1:100).
    Parsons, M.P. and Hirasawa, M. (2010) J. Neurosci. 30, 8061.
  7. Rat brain and heart sections (1:500).
    Zoga, V. et al. (2010) Mol. Pain 6, 1.
More product citations
  1. Tajada, S. et al. (2012) J. Physiol. 590, 6075.
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