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Anti-KISS1 Receptor (GPR54) (extracellular) Antibody

Kisspeptin receptor, KISS1R, Metastin receptor, AXOR12

Cat #: AKR-001
Alternative Name Kisspeptin receptor, KISS1R, Metastin receptor, AXOR12
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, r
May also work in: m*
Immunogen
  • Peptide (C)GSWHPRSYAAYALK, corresponding to amino acid residues 292-305 of human KISS1R (Accession Q969F8). 3rd extracellular loop.
Accession (Uniprot) Number Q969F8
Gene ID 84634
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - 13/14 amino acid residues identical; mouse - 12/14 amino acid residues identical.
RRID AB_2039851.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.75 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of Jurkat (lanes 1 and 4), HL-60 (lanes 2 and 5), MCF-7 (lanes 3 and 6) and rat brain (lanes 7 and 8) lysates:
    Western blot analysis of Jurkat (lanes 1 and 4), HL-60 (lanes 2 and 5), MCF-7 (lanes 3 and 6) and rat brain (lanes 7 and 8) lysates:
    1,2,3,7. Anti-KISS1 Receptor (GPR54) (extracellular) Antibody (#AKR-001), (1:500).
    4,5,6,8. Anti-KISS1 Receptor (GPR54) (extracellular) Antibody, preincubated with KISS1 Receptor/GPR54 (extracellular) Blocking Peptide (#BLP-KR001).
Immunohistochemistry
  • Expression of KISS1R in rat pancreas
    Expression of KISS1R in rat pancreas
    Immunohistochemical staining of paraffin embedded section of rat pancreas using Anti-KISS1 Receptor (GPR54) (extracellular) Antibody (#AKR-001), (1:100). KISS1R staining (brown) appears in Isles of Langerhans (IL). Hematoxilin is used as the counterstain.
  • Expression of KISS1R in rat cerebellum
    Expression of KISS1R in rat cerebellum
    Immunohistochemical staining of frozen rat cerebellum section using Anti-KISS1 Receptor (GPR54) (extracellular) Antibody (#AKR-001), (1:100). A. KISS1R (green) was expressed particularly in Purkinje cell bodies (arrows). B. Staining with mouse anti-parvalbumin (red) detected Purkinje cells and interneurons in the molecular layer. C. Merge of the two images demonstrates that the staining was restricted to Purkinje cells.
Indirect flow cytometry
  • Cell surface detection of KISS1 Receptor by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells:
    Cell surface detection of KISS1 Receptor by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-KISS1 Receptor (GPR54) (extracellular) Antibody (#AKR-001), (5μg) + goat-anti-rabbit-FITC.
References
  1. IUPHAR DATABASE | KiSS1-derived peptide receptor.
  2. Kauffman, A.S. et al. (2007) J. Neurosci. 27, 8826.
  3. Dungan, H.M. et al. (2006) Endocrinology 147, 1154.
Scientific background

The kisspeptin receptor (KISS1R, GPCR54) is a member of the G-protein coupled receptor superfamily. KISS1R natural ligands are peptides called kisspeptins which are encoded by the Kiss1 gene. The Kiss1 gene was originally discovered by Hershey scientists who named it after their famous chocolate, the “Kiss”.

The kisspeptins, acting through KISS1R, were found to be potent inhibitors of metastasis in a variety of cancer cell types including thyroid and pancreatic cancer cells. KISS1R and kisspeptins are involved in the direct regulation of the reproductive neuroendocrine axis.

KISS1R is widely distributed throughout the brain, where it is most highly expressed in the hypothalamic and amygdaloid nuclei. High expression was also found in the pancreas and placenta. Lower-level expression was found in a variety of other peripheral tissues including peripheral blood leukocytes, kidney, lung, testes, spleen, thymus, adrenal glands and lymph nodes.1-3

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-KISS1 Receptor (GPR54) (extracellular) Antibody (#AKR-001) is a highly specific antibody directed against an epitope of the human kisspeptin receptor. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KISS1R from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Rat brain lysates (1:500).
    Jia, Y.F. et al. (2017) Int. J. Clin. Exp. Pathol. 10, 6099.
  2. Human primary mesenchymal stem cells (MSCs).
    Dotterweich, J. et al. (2016) PLoS ONE 11, e0155087.
Immunohistochemistry citations
  1. Canine ovary sections (1:200).
    Cielesh, M. et al. (2017) Reprod. Domest. Anim. 52, 211.
  2. Rat brain sections (1:500).
    Jia, Y.F. et al. (2017) Int. J. Clin. Exp. Pathol. 10, 6099.
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