- Peptide KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3 (Accession P22001). 1st extracellular loop.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101-GP), (1:200).
3,4. Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).
- Expression of KV1.3 in mouse cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101-GP), (1:100). KV1.3 staining (red) is detected in Bergmann glia outlines (arrow). DAPI is used as the counterstain (blue) .
KV1.3 belongs to the Shaker family of voltage-dependent K+ channels. The channel, encoded by KCNA3, is widely expressed in the brain, lung and osteoclasts and in several cell populations of hematopoietic origin. The prominence of KV1.3 channels in these cells (particularly in T lymphocytes) directed much research attention. It was found that KV1.3 is the main channel responsible for maintaining the resting potential in quiescent cells and regulating the Ca2+ signaling that is indispensable for normal T lymphocyte activation.1,2 Based on the central role of KV1.3 in regulating the initiation of an immune response, the channel has been recognized as a potential target for immunosuppressant drugs.1
Species reactivity key:
Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101-GP) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KV1.3 potassium channel from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.