Name: Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody
Brand: Alomone Labs
SKU: APC-101-GP (formerly AGP-005)

Overview

Cat #: APC-101-GP (formerly AGP-005)

Alternative Name Potassium voltage-gated channel subfamily A member 3

Lyophilized Powder yes

Type: Polyclonal

Host: Guinea pig

Reactivity: h, m, r

Immunogen

Peptide KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human K_V1.3 (Accession P22001). 1^st extracellular loop.

Accession (Uniprot) Number P22001

Gene ID 3738

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, mouse - 12/14 amino acid residues identical.

RRID AB_2340958.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Guinea pig total IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*, lci*

Western blot

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
1,2. Guinea pig Anti-K_V1.3 (KCNA3) (extracellular) Antibody (#APC-101-GP), (1:200).
3,4. Guinea pig Anti-K_V1.3 (KCNA3) (extracellular) Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).

Immunohistochemistry

Expression of K_V1.3 in mouse cerebellum
Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Guinea pig Anti-K_V1.3 (KCNA3) (extracellular) Antibody (#APC-101-GP), (1:100). K_V1.3 staining (red) is detected in Bergmann glia outlines (arrow). DAPI is used as the counterstain (blue) .

References

Chandy, K.G. et al. (2001) Toxicon 39, 1269.
Koo, G.C. et al. (1997) J. Immunol. 158, 5120.

Scientific background

K_V1.3 belongs to the Shaker family of voltage-dependent K⁺ channels. The channel, encoded by KCNA3, is widely expressed in the brain, lung and osteoclasts and in several cell populations of hematopoietic origin. The prominence of K_V1.3 channels in these cells (particularly in T lymphocytes) directed much research attention. It was found that K_V1.3 is the main channel responsible for maintaining the resting potential in quiescent cells and regulating the Ca²⁺ signaling that is indispensable for normal T lymphocyte activation.^1,2 Based on the central role of K_V1.3 in regulating the initiation of an immune response, the channel has been recognized as a potential target for immunosuppressant drugs.¹

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Guinea pig Anti-K_V1.3 (KCNA3) (extracellular) Antibody (#APC-101-GP) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize K_V1.3 potassium channel from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-K_V1.3 (KCNA3) (extracellular) Antibody (#APC-101) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 07/08/2023

Applications

Citations

Immunohistochemistry citations

Human head and neck tumor sections.
Chimote, A.A. et al. (2017) Cancer Res. 77, 53.

Specifications

Scientific Background

Specific Control Product

Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101) is the original antigen used for immunization during Anti-K_V1.3 (KCNA3) (extracellular) Antibody (#APC-101) generation. The blocking peptide binds and ‘blocks’ Anti-Kv1.3/KCNA3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101)

Shipping and Ordering Information

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Guinea pig Anti-K_V1.3 (KCNA3) (extracellular) Antibody