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Anti-KV1.3 (KCNA3) (extracellular)-FITC Antibody

Potassium voltage-gated channel subfamily A member 3

Cat #: APC-101-F
Alternative Name Potassium voltage-gated channel subfamily A member 3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3 (Accession P22001). Extracellular loop between domains S1 and S2.
Accession (Uniprot) Number P22001
Gene ID 3738
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse - 12/14 amino acid residues identical.
RRID AB_2040147.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label Fluorescein isothiocyanate (FITC).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #APC-101), and direct flow cytometry (labeled antibody).
Applications: fc, ic, if, lci
Direct flow cytometry
  • Cell surface detection of KV1.3 in live intact Jurkat (human T cell leukemia) cells:
    Cell surface detection of KV1.3 in live intact Jurkat (human T cell leukemia) cells:
    ___ Unstained cells.
    ___ Cells + Anti-KV1.3 (KCNA3) (extracellular)-FITC Antibody (#APC-101-F), (0.5-5 µg antibody / 1x106 cells).
  • The control antigen is not suitable for this application.
Live cell imaging / Immunocytochemistry
  • Mouse living primary cortical cells (Duque, A. et al. (2013) Develop. Neurobiol. 73, 841.).
References
  1. Chandy, K.G. et al. (2001) Toxicon 39, 1269.
  2. Koo, G.C. et al. (1997) J. Immunol. 158, 5120.
  3. Grissmer, S. et al. (1994) Mol. Pharmacol. 45, 1227.
  4. Garcia-Calvo, M. et al. (1993) J. Biol. Chem. 268, 18866
  5. Garcia, M.L. et al. (1994) Biochemistry 33, 6834.
  6. Koschak, A. et al. (1998) J. Biol. Chem. 273, 2639.
  7. Kalman, K. et al. (1998) J. Biol. Chem. 273, 32697.
Scientific background

KV1.3 belongs to the Shaker family of voltage-dependent K+ channels. The channel, encoded by KCNA3, is widely expressed in the brain, lung and osteoclasts and in several cell populations of hematopoietic origin. The prominence of KV1.3 channels in these cells (particularly in T lymphocytes) directed much research attention. It was found that KV1.3 is the main channel responsible for maintaining the resting potential in quiescent cells and regulating the Ca2+ signaling that is indispensable for normal T lymphocyte activation.1,2 Based on the central role of KV1.3 in regulating the initiation of an immune response, the channel has been recognized as a potential target for immunosuppressant drugs.1

KV1.3 channels are potently inhibited by several venomous peptide toxins among them Charybdotoxin (2.6 nM), Noxiustoxin (1 nM), Kaliotoxin (0.65 nM), Margatoxin (0.05 nM), Agitoxin-1 (1.7 nM), Agitoxin-2 (0.004 nM), Hongotoxin-1 (0.09 nM)  and Stichodactyla toxin (0.01 nM).3-7

The central role of KV1.3 in immune system cells created a real need for a specific antibody that would be able to work in flow cytometry applications.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
Last update: 12/08/2021

Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and indirect flow cytometry applications. It has been designed to recognize KV1.3 potassium channel from human, mouse, and rat samples.

Anti-KV1.3 (KCNA3) (extracellular)-FITC Antibody (#APC-101-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

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For research purposes only, not for human use

Applications

Citations

Citations
Live cell imaging citations
  1. Mouse primary cortical cells.
    Duque, A. et al. (2013) Develop. Neurobiol. 73, 841.
Direct flow cytometry citations
  1. Human acute lymphoid (CEM) and myeloid  (AML-3) leukemia cell line.
    Lowinus, T. et al. (2019) Cell Commun. Signal. 17, 5.
More product citations
  1. Gilhar, A. et al. (2012) J. Invest. Dermatol. 131, 118.

Specifications

Scientific Background

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