Anti-KVβ1 Antibody

Voltage-gated potassium channel subunit beta-1, KV beta 1, KVb1, KCNAB1
Cat #: APC-127
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)TPQHHISLKESTAK, corresponding to amino acid residues 54-67 of rat KCNAB1 (Accession P63144). N-terminus.
    Accession (Uniprot) Number P63144
    Gene ID 29737
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity This antibody will recognize the "canonical" splice isoform of human KVβ1 (also known as KVβ1.3). It will not recognize splice variants KVβ1.1 and KVβ1.2. For more information see human KVβ1 entry Q14722.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic, ifc, ih, ip
    Western blot
    Western blot analysis of human SH-SY5Y neuroblastoma cell lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and rat brain lysate (lanes 3 and 6):
    1-3. Anti-KVβ1 Antibody (#APC-127), (1:200).
    4-6. Anti-KVβ1 Antibody, preincubated with the control peptide antigen.
    References
    1. Hille, B. (1992) Sinauer, Sunderland, MA.
    2. Zagotta, W.N. et al. (1990) Science 250, 56.
    3. Pongs, O. et al. (1999) Ann. N. Y. Acad. Sci. 868, 344.
    4. McCormack, K. et al. (1995) FEBS Lett. 370, 32.
    Scientific background

    Voltage-gated K+ channels represent a structurally and functionally diverse group of membrane proteins. These channels establish the resting membrane potential and modulate the frequency and duration of action potentials in nerve and muscle1. The duration of the open states of K+ channels is determined by the rates of deactivation and inactivation. KV channels are hetero-oligomeric complexes consisting of two different types of subunits: membrane-bound, pore-forming α subunits and the smaller β subunits. The β subunits appear to be peripheral proteins tightly associated with the cytoplasmic side of the α subunits2

    The inactivation gate can be formed by an N-terminal segment of an auxiliary β-subunit, as in the complex of KVβ1 and KVα family channels3. There are three mammalian KVβ genes: KVβ1, KVβ2 and KVβ3. Additional variability in the KVβ1 family results from alternative splicing in the amino terminal region, thus yielding the KVβ1.1, KVβ1.2, and KVβ1.3 subunits4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 13/03/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KVβ1. Anti-KVβ1 Antibody (#APC-127) can be used in western blot analysis. This antibody will recognize the “canonical” splice isoform of human KVβ1 (also known as KVβ1.3). It won’t recognize splice variants KVβ1.1 and KVβ1.2. For more information see human KVβ1 entry Q14722. It has been designed to recognize KVβ1 from rat, mouse and human samples.

    For research purposes only, not for human use