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Anti-KCNH1 (EAG-1) Antibody

KV10.1, Ether-a-go-go potassium channel 1, hEAG1

Cat #: APC-104
Alternative Name KV10.1, Ether-a-go-go potassium channel 1, hEAG1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide GDPAKRKGWARFKDAC, corresponding to amino acid residues 802-817 of rat KCNH1 (Accession Q63472). Intracellular, C-terminus.
Accession (Uniprot) Number Q63472
Gene ID 65198
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human -14/16 amino acid residues identical.
RRID AB_2039931.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity The antibody is specific for KV10.1 and will not cross-react with KV10.2.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.6 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, ip, wb
May also work in: ifc*
Western blot
  • Western blot analysis of rat brain lysate (lanes 1 and 3) and HEK-KCNH1 (lanes 2 and 4):
    Western blot analysis of rat brain lysate (lanes 1 and 3) and HEK-KCNH1 (lanes 2 and 4):
    1,2. Anti-KCNH1 (EAG-1) Antibody (#APC-104), (1:200).
    3,4. Anti-KCNH1 (EAG-1) Antibody, preincubated with KCNH1/EAG-1 Blocking Peptide (#BLP-PC104).
  • Mouse brain and colon lysates (Ousingsawat, J. et al. (2007) Clin. Cancer Res. 13, 824.).
Immunoprecipitation
  • HEK 293T transfected cells (Hsu, P.H. et al. (2016) FEBS Open Bio 6, 349.).
Immunohistochemistry
  • Paraffin embedded human gliomas (1:200) (Bai, Y. et al. (2013) Eur. J. Cancer 49, 710.).
Immunocytochemistry
  • Expression of EAG1 in human MDA-468 mammary gland adenocarcinoma cells.
    Expression of EAG1 in human MDA-468 mammary gland adenocarcinoma cells.
    Immunocytochemical staining of human MDA-468 mammary gland adenocarcinoma cells. A. Cells were stained with Anti-KCNH1 (EAG-1) Antibody (#APC-104), (1:200) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red). B. Nuclei were visualized with the cell-permeable dye Hoechst 33342 (blue). C. Merged image of panels A and B.
References
  1. Ludwig, J. et al. (1994) EMBO J. 13, 4451.
  2. Bauer, C.K. et al. (2001) J. Membrane Biol. 182, 1.
  3. Pardo, L.A. et al. (1999) EMBO J. 18, 5540.
Scientific background

KV10.1 is a member of the Ether-a-go-go family of voltage-activated potassium channels. To date, the family includes eight members: the closely related KV10.2 (EAG-2) protein, the KV12 (elk) (elk1, elk2, elk3) and the KV11 (erg) proteins (erg1, erg2, erg3). As with the other KV superfamily members, KV10.1 shows the characteristic 6 transmembrane domains with intracelullar N- and C-termini.

The KV10 subfamily current is characterized by outward rectification without inactivation and slow activation kinetics. Its expression is normally confined to the brain where its physiological function has not yet been clarified.

Intriguingly, the KV10.1 channel has been implicated in malignant tumor development. It has been shown that KV10.1 was inappropriately expressed in several cancer cell lines. Moreover, one study showed that KV10.1 by itself had oncogenic potential, as a cell line transfected with the channel, induced aggressive and faster tumor growth in vivo as compared to a cell line transfected with an unrelated KV channel.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat KV10.1 channel. Anti-KCNH1 (EAG-1) Antibody (#APC-104) can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KV10.1 from rat, human, and mouse samples. The antibody is specific for KV10.1 and will not cross-react with KV10.2.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. HEK-293T transfected cells.
    Hsu, P.H. et al. (2016) FEBS Open Bio 6, 349.
Immunoprecipitation citations
  1. HEK-293T transfected cells.
    Hsu, P.H. et al. (2016) FEBS Open Bio 6, 349.
Immunohistochemistry citations
  1. Human breast cancer tissue sections.
    Peretti, M. et al. (2019) Sci. Rep. 9, 1175.
Immunocytochemistry citations
  1. Human MCF-7 cells.
    Peretti, M. et al. (2019) Sci. Rep. 9, 1175.
More product citations
  1. Bai, Y. et al. (2013) Eur. J. Cancer 49, 710.
  2. Spitzner, M. et al. (2008) J. Biol. Chem. 283, 7421.
  3. Ousingsawat, J. et al. (2007) Clin. Cancer Res. 13, 824.
  4. Spitzner, M. et al. (2007) FASEB J. 21, 35.
  5. Cherubini, A. et al. (2005) Mol. Biol. Cell 16, 2972.
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