Anti-KV12.3 (KCNH4) Antibody

Potassium voltage-gated channel subfamily H member 4, Ether-a-go-go-like potassium channel 1, ELK channel 1, rElk1, Elk3, Brain-specific eag-like channel 2, BEC2
    Cat #: APC-116
    Alternative Name Potassium voltage-gated channel subfamily H member 4, Ether-a-go-go-like potassium channel 1, ELK channel 1, rElk1, Elk3, Brain-specific eag-like channel 2, BEC2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, r
      • Peptide CRLNQEISRLNQEVS, corresponding to amino acids 881-895 of rat KCNH4 (Accession Q9R1T9). Intracellular, C-terminal part.
        Anti-KV12.3 (KCNH4) Antibody
    Accession (Uniprot) Number Q9R1T9
    Gene ID 114032
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human - identical.
    RRID AB_2039933.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic*, ifc*, ip*
      • Anti-KV12.3 (KCNH4) Antibody
        Western blot analysis of rat brain (lanes 1 and 3) and cerebellum (lanes 2 and 4) lysates:
        1,2. Anti-KV12.3 (KCNH4) Antibody (#APC-116), (1:200).
        3,4. Anti-KV12.3 (KCNH4) Antibody, preincubated with the control peptide antigen.
      • Anti-KV12.3 (KCNH4) Antibody
        Expression of KV12.3 (Elk3) in rat testes
        Immunohistochemical staining of rat testes paraffin embedded section using Anti-KV12.3 (KCNH4) Antibody (#APC-116), (1:100).
        KV12.3 (pink staining) is expressed in the columnar epithelium of the epididymus. Hematoxilin is used as the counterstain.
    References
      • KV12.3 (also known as KCNH4, Elk3) is a voltage-gated K+ channel that belongs to the ether-a-go-go (EAG) family. The EAG family of voltage-gated K+ channels can be subdivided into three distinct groups based on sequence homology. These are the eag (KV10) with two members, the eag-related channels (erg or KV11) and the eag-like K+ channels (elk or KV12) with three members each.

        As with all members of the family, KV12.3 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N- and C- termini. The functional channel is a tetramer composed of four subunits. It has been suggested that the KV12 subfamily members can form functional heteromultimers within the subfamily.1,2

        KV12.3 channel distribution appears to be mainly confined to the central nervous system with some expression in peripheral organs such as the testis and lung.3

        The physiological function of the KV12.3 channel is yet to be established, although a role in the modulation of overall excitability of neurons has been suggested.3  

        Currently, there isn’t much pharmacological data concerning the modulation of the KV12.3 channel except for blocking of the channel by Ba2+.3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the intracellular C-terminal end of the rat KV12.3. Anti-KV12.3 (KCNH4) Antibody (#APC-116) can be used in western blot and immunohistochemical applications, and recognizes KV12.3 from rat and human samples.

    For research purposes only, not for human use