Anti-KVβ2 Antibody

Voltage-gated K+ channel β subunit 2, KCNAB2
    Cat #: APC-117
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide SPGMIYSTRYGSPKR(C), corresponding to amino acid residues 20-34 of rat Kvβ2 (Accession P62483).  N-terminal part.
    Accession (Uniprot) Number P62483
    Gene ID 29738
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human, rabbit - identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, wb
    May also work in: ifc, ip
    Western blot
    Western blot analysis of rat brain lysate (lanes 1 and 3) and membranes (lanes 2 and 4):
    1, 2. Anti-Kvβ2 Antibody (#APC-117), (1:200).
    3, 4.  Anti-Kvβ2 Antibody, preincubated with the control peptide antigen.
    Western blot analysis of human Jurkat T cells:
    1.  Anti-Kvβ2 Antibody (#APC-117), (1:200).
    2.  Anti-Kvβ2 Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of Kvβ2 in mouse cerebellum
    Immunohistochemical staining of mouse cerebellum using Anti-Kvβ2 Antibody (#APC-117). A. Kvβ2 appears adjacent to Purkinje cells (green). B. Staining of GABAergic cells with mouse anti parvalbumin (PV, red). C. Confocal merge of Kvβ2 and PV demonstrates presence of Kvβ2 adjacent to Purkinje cells.
    Immunocytochemistry
    Rat hippocampus primary neurons (1:200) (Proepper, C. et al. (2014) Neuroscience 261, 133.).
    References
    1. Parcej, D.N. et al.  (1992) Biochemistry 31, 11084.
    2. Rettig, J. et al. (1994) Nature 369, 289.
    3. Pongs, O. et al. (1999) Ann. N. Y. Acad. Sci. 868, 344.
    4. Li, Y. et al. (2006) Neuroscientist 12, 199.
    Scientific background

    KVβ2 (or KCNAB2) is a member of a family of proteins that regulate the activity of voltage-dependent K+ channels. The other members of the family are KVβ1 and KVβ3.

    The KVβ subunits were originally identified using a biochemical approach that demonstrated that the ion-conducting a subunits existed in a macromolecular complex with auxiliary β subunits with a probable stoichiometry of α4β4. It is now widely established that the regulatory β subunits are able to alter both the biophysical (i.e. acceleration of inactivation kinetics) and biochemical (promote cell surface expression) properties of the functional KV channel.

    The KVβ regulatory subunits are cytosolic proteins with conserved C-termini and variable N-terminus domains. The interaction with the a subunits is via the conserved C-terminal end that binds to a specific sequence in the N-terminus domain of the a subunits.

    KVβ2, as well as the other KVβ subunits can bind to all the members of the large KV1.x (Shaker) channel subfamily.  However, recent evidence suggests that it can also bind to members of the KV4.x subfamily.

    KVβ2 is the most abundant β subunit in the brain where it couples with all the Kv1.x subunits, but the protein is also expressed in peripheral tissues including heart, lung and cells of hematopoietic origin such as T cells where it couples with KV1.3, the dominant voltage-dependent K+ channel in these cells.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 01/11/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KVβ2. Anti-KVβ2 Antibody (#APC-117) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize KVβ2 from rat, mouse and human samples.

    For research purposes only, not for human use
    Citations
    Western blot citations
    1. Rat brain lysate (1:400).
      Proepper, C. et al. (2014) Neuroscience 261, 133.
    Immunocytochemistry citations
    1. Rat hippocampus primary neurons (1:200).
      Proepper, C. et al. (2014) Neuroscience 261, 133.
    More product citations
    1. Jukkola, P.I. et al. (2012) Neurobiol. Dis. 47, 280.
    2. Zencker, J. et al. (2012) J. Neurosci. 32, 7493.
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