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Anti-KCNC3 (KV3.3) Antibody

Potassium voltage-gated channel subfamily C member 3, KSHIIID, SCA13

Cat #: APC-102
Alternative Name Potassium voltage-gated channel subfamily C member 3, KSHIIID, SCA13
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide KSPITPGSRGRYSRDRAC, corresponding to amino acid residues 701-718 of rat KCNC3 (Accession Q01956). Intracellular, C-terminus.
    Accession (Uniprot) Number Q01956
    Gene ID Q01956
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human, mouse-identical.
    RRID AB_2040170.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity Recognizes only splice variant KV3.3-1.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
    Western blot
    • Western blotting of rat brain membranes: 
      Western blotting of rat brain membranes: 
      1. Anti-KCNC3 (KV3.3) Antibody (#APC-102), (1:200).  
      2. Anti-KCNC3 (KV3.3) Antibody, preincubated with KCNC3/Kv3.3 Blocking Peptide (#BLP-PC102).
    Immunohistochemistry
    • Rat brain sections.
    Immunocytochemistry
    References
    1. Rudy, B. and McBain C.J. (2001) Trends Neurosci. 24, 517.
    2. Matsukawa, H. et al. (2003) J Neurosci. 23, 7677.
    Scientific background

    KV3.3 is a member of the Shaw-type family of voltage-gated K+ channels that includes four members (KV3.1-3.4). 

    KV3 subfamily members inactivate very rapidly and therefore are thought to play a role in the repolarization of action potentials and to facilitate repetitive high frequency firing.  

    KV3.3 is mostly localized in the brain, although it has been observed in vascular smooth muscle cells and eye epithelium. In the brain its expression pattern largely overlaps that of the KV3.1 channel suggesting that they may form functional heteromers. Indeed, mouse knockouts of both KV3.1 and KV3.3, but not either channel alone, display severe motor defects.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 12/08/2021

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat KV3.3 channel. Anti-KCNC3 (KV3.3) Antibody (#APC-102) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize KV3.3 from rat, mouse and human samples.

    For research purposes only, not for human use

    Applications

    Citations

    Citations
    KO validation citations
    1. Western blot of mouse brainstem samples and immunohistochemistry of mouse medial nucleus of the trapezoid body (MNTB). Tested in Kv3.3 knockout mice.
      Choudhury, N. et al. (2020) J. Physiol. 598.11, 2199.
    Immunocytochemistry citations
    1. Mouse spiral ganglia (SGNs).
      Chen, W.C. and Davis R.L. (2006) Hear. Res. 222, 89.
    More product citations
    1. Alonso-Espinaco, V. et al. (2008) Neuroscience 155, 1059.
    2. Hurlock, E.C. et al. (2008) J. Neurosci. 28, 4640.
    3. Cartwright, T. A. et al. (2007) Biochim. Biophys. Acta 1770, 666.
    4. Kaneda, M. et al. (2007) J. Neurophysiol. 97, 4225.
    5. Pannaccione, A. et al. (2007) Mol. Pharmacol. 72, 665.
    6. Xia, F. et al. (2007) Endocrinol. 148, 2157.
    7. Brooke, R.E. et al. (2004) Eur. J. Neurosci. 20, 3313.
    8. Shevchenko, T. et al. (2004) J. Neurophysiol. 92, 3043.

    Specifications

    Scientific Background

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