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Anti-LPAR3 (EDG7) (extracellular) Antibody

Lysophosphatidic acid receptor 3, LPA receptor 3, LPA-3, snGPCR32

Cat #: ALR-033
Alternative Name Lysophosphatidic acid receptor 3, LPA receptor 3, LPA-3, snGPCR32
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide-(C)RSNTDTADEWTGTK, corresponding to amino acid residues 16-29 of rat LPA3 receptor (Accession Q8K5E0). Extracellular, N-terminus.
Accession (Uniprot) Number Q8K5E0
Gene ID 66025
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 12/14 amino acid residues identical.
RRID AB_2340991.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*, lci*
Western blot
  • Western blot analysis of rat testis (lanes 1 and 3) and human OVCAR-3 (lanes 2 and 4):
    Western blot analysis of rat testis (lanes 1 and 3) and human OVCAR-3 (lanes 2 and 4):
    1,2. Anti-LPAR3 (EDG7) (extracellular) Antibody (#ALR-033), (1:200).
    3,4. Anti-LPAR3 (EDG7) (extracellular) Antibody, preincubated with LPAR3/EDG7 (extracellular) Blocking Peptide (#BLP-LR033).
References
  1. Gendaszewska-Darmach, E. et al. (2008) Acta Biochim. Pol. 55, 227.
  2. Noguchi, K. et al. (2003) J. Biol. Chem. 278, 25600.
  3. Choi, J.W. et al. (2010) Annu. Rev. Pharmacol. Toxicol. 50, 157.
  4. Oh, D.Y. et al. (2006) Int. Rev. Cytol. 252, 163.
  5. Bandoh, K. et al. (1999) J. Biol. Chem. 274, 27776.
  6. Contos, J.J. et al. (2002) Mol. Cell. Biol. 22, 6921.
  7. Ye, X. et al. (2005) Nature 435, 104.
Scientific background

Lysophosphatidic acid (LPA) is a bioactive lipid molecule with a phosphate, a glycerol, and a fatty acid in its structure. The cellular effects of LPA include proliferation, migration, cytokine secretion, and morphological change. These pleiotropic actions allow LPA to participate in a wide variety of biological processes, such as brain development, oncogenesis, wound healing and cancer progression1. To date, at least 6 subtypes of LPA receptors have been identified. The LPA1-3 receptors are members of the endothelial cell differentiation gene (Edg) family2. All six receptors are type I, rhodopsin-like G-protein coupled receptors (GPCRs) that differ in their tissue distribution and downstream signaling pathways3.

GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs); the N-terminus is on the extracellular side of the membrane, and the C-terminus is on the cytoplasmic side4.

Expression of LPA3 receptor has been observed in human heart, testis, prostate, pancreas, lung, ovary, and brain5 and is most abundant in mouse testis, kidney, lung, small intestine, heart, stomach, spleen, brain, and thymus6.

LPA3 receptor null mice are viable and grossly normal, but female nulls show a striking phenotype in the reproductive system7. However, despite the fact that LPA3 is expressed in the frontal cortex, hippocampus, and amygdala, no phenotypes related to LPA3 loss in the nervous system have been reported to date5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/06/2021

Anti-LPAR3 (EDG7) (extracellular) Antibody (#ALR-033) is a highly specific antibody directed against an epitope to the rat LPA3 receptor. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize LPA3 receptor from mouse, rat, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Human dermal fibroblasts cell lysate (1:1000).
    Olianas, M.C. et al. (2020) Eur. J. Pharmacol. 873, 172963.
  2. Rat C6 glioma cell lysate.
    Olianas, M.C. et al. (2016) J. Pharmacol. Exp. Ther. 359, 340.
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