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Anti-LPAR6 (P2RY5) (extracellular) Antibody

Lysophosphatidic acid receptor 6, LPA receptor 6, LPA-6, P2Y purinoceptor 5, Purinergic receptor 5, P2Y5, RB intron encoded G-protein coupled receptor

Cat #: ALR-036
Alternative Name Lysophosphatidic acid receptor 6, LPA receptor 6, LPA-6, P2Y purinoceptor 5, Purinergic receptor 5, P2Y5, RB intron encoded G-protein coupled receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide STHSQGNNASEAC, corresponding to amino acid residues 156-168 of human LPA receptor 6 (Accession P43657). 2nd extracellular loop.
Accession (Uniprot) Number P43657
Gene ID 10161
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse -12/13 amino acid residues identical.
RRID AB_2340993.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, lci, wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
    1,2. Anti-LPAR6 (P2RY5) (extracellular) Antibody (#ALR-036), (1:200).
    3,4. Anti-LPAR6 (P2RY5) (extracellular) Antibody, preincubated with LPAR6/P2RY5 (extracellular) Blocking Peptide (#BLP-LR036).
Indirect flow cytometry
  • Cell surface detection of LPAR6 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: 
    Cell surface detection of LPAR6 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: 
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-LPAR6 (P2RY5) (extracellular) Antibody (#ALR-036) , (2.5μg) + goat-anti-rabbit-FITC.
References
  1. Gendaszewska-Darmach, E. et al. (2008) Acta Biochim. Pol. 55, 227.
  2. Noguchi, K. et al. (2003) J. Biol. Chem. 278, 25600.
  3. Yanagida, K. et al. (2009) J. Biochem. 284, 17731.
  4. Oh, D.Y. et al. (2006) Int. Rev. Cytol. 252, 163.
  5. Pasternack, S.M. et al. (2010) Nat. Genet. 40, 329.
Scientific background

Lysophosphatidic acid (LPA; 1- or 2-acyl-sn-glycerol-3-phosphate) is a bioactive lipid molecule with a phosphate, a glycerol, and a fatty acid in its structure. The cellular effects of LPA include proliferation, migration, cytokine secretion, and morphological change. These pleiotropic actions allow LPA to participate in a wide variety of biological processes, such as brain development, oncogenesis, wound healing and cancer progression1. To date, at least 6 subtypes of LPA receptor have been identified. The LPA1-3 receptors are members of the endothelial cell differentiation gene (Edg) family2.

LPA6 (P2Y5) was cloned as an orphan GPCR and is closely related to the fourth lysophosphatidic acid receptor, LPA43. GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs); the N terminus is on the extracellular side of the membrane, and the C terminus is on the cytoplasmic side4.

LPA6 mRNA is expressed ubiquitously including in skin and hair follicle cells.

A recent study suggested that LPA6 is essential for human hair growth. A loss of function is not compensated for, and ultimately leads to pathological changes and hair loss5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 28/07/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human LPA receptor 6. Anti-LPAR6 (P2RY5) (extracellular) Antibody (#ALR-036) can be used in western blot analysis and indirect flow cytometry. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize LPA receptor 6 from mouse, rat and human samples.

For research purposes only, not for human use
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