Name: Anti-LRRC8A (extracellular) Antibody
Brand: Alomone Labs
SKU: AAC-001

Overview

Cat #: AAC-001

Alternative Name Leucine-rich repeat-containing protein 8A, Volume-regulated anion channel subunit LRRC8A, LRRC8, Swelling protein 1, SWELL1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)DTGPTGIKYDLDRH, corresponding to amino acid residues 91-104 of human LRRC8A (Accession Q8IWT6). 1^st extracellular loop.

Accession (Uniprot) Number Q8IWT6

Gene ID 56262

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, mouse – identical.

RRID AB_2756534.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ifc, ih, lci, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of mouse brain lysate:
1. Anti-LRRC8A (extracellular) Antibody (#AAC-001), (1:200).
2. Anti-LRRC8A (extracellular) Antibody, preincubated with LRRC8A (extracellular) Blocking Peptide (#BLP-AC001).
Western blot analysis of rat lung membranes:
1. Anti-LRRC8A (extracellular) Antibody (#AAC-001), (1:200).
2. Anti-LRRC8A (extracellular) Antibody, preincubated with LRRC8A (extracellular) Blocking Peptide (#BLP-AC001).

Immunohistochemistry

Expression of LRRC8A in mouse cerebellum and olfactory bulb
Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-LRRC8A (extracellular) Antibody (#AAC-001), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. LRRC8A staining in mouse cerebellum (green) shows intensely positive cells (arrows) with Bergmann glia morphology and localization. B. Staining in mouse olfactory bulb shows intensely positive cells (arrows) with glial morphology in some glomeruli (G). Cell nuclei are stained with DAPI (blue).

Indirect flow cytometry

Human histiocytic lymphoma U937 cells (Yurinskaya, V. et al. (2020) Channels 14, 45.).
Cell surface detection of LRRC8A by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-LRRC8A (extracellular) Antibody (#AAC-001), (5μg) + goat-anti-rabbit-FITC.

References

Wang, R. et al. (2017) JCI Insight 2, e90632.
Lutter, D. et al. (2017) J. Cell. Sci.130, 1122.
Gradogna, A. et al. (2017) Channels (Austin) 11, 254.

Scientific background

Leucine-rich repeat-containing 8 A (LRRC8A, SWELL1) is an essential component of the volume-regulated anion channel (VRAC) that is activated by cell swelling and ionic strength. In response to swelling, mammalian cells release chloride and organic osmolytes through VRAC channels. LRRC8A serves as a sensor of ionic strength gradient in the absence of osmotic gradient. In addition, LRRC8A plays a role in the uptake of chemotherapeutic agents^1-3.

LRRC8A protein structure contains 4 transmembrane segments and about 17 leucine-rich repeats (LRRs) at the cytoplasmic C-terminus³. VRAC channels are composed of the LRRC8A subunit and at least one of LRRC8B-E subunits. Deletion of LRRC8A greatly decreases the hypo-osmolarity-induced current^1,3.

LRRC8A mRNA and protein are expressed in several neuronal tissues including brain stem ganglia, nodose ganglia and dorsal root ganglia. LRRC8A is also detected in the heart, aorta, and small intestine¹.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-LRRC8A (extracellular) Antibody (#AAC-001) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize SWELL1 from rat, mouse, and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 03/01/2024

Applications

Citations

Indirect flow cytometry citations

Human histiocytic lymphoma U937 cells.
Yurinskaya, V. et al. (2020) Channels 14, 45.

Specifications

Scientific Background

Specific Control Product

LRRC8A (extracellular) Blocking Peptide (#BLP-AC001) is the original antigen used for immunization during Anti-LRRC8A (extracellular) Antibody (#AAC-001) generation. The blocking peptide binds and ‘blocks’ Anti-LRRC8A (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

LRRC8A (extracellular) Blocking Peptide (#BLP-AC001)

Shipping and Ordering Information

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Anti-LRRC8A (extracellular) Antibody