Anti-CHRM1 (443-458) Antibody

Cholinergic muscarinic receptor 1, Muscarinic acetylcholine receptor M1
    Cat #: AMR-010
    Alternative Name Cholinergic muscarinic receptor 1, Muscarinic acetylcholine receptor M1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)RKIPKRPGSVHRTPSR, corresponding to amino acid residues 443-458 of human M1 muscarinic receptor (Accession P11229). Intracellular, C-terminus.
        Anti-CHRM1 (443-458) Antibody
    Accession (Uniprot) Number P11229
    Gene ID 1128
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat - identical.
    RRID AB_2340994.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
      • Anti-CHRM1 (443-458) Antibody
        Western blot analysis of rat brain lysate:
        1. Anti-CHRM1 (443-458) Antibody (#AMR-010), (1:200).
        2. Anti-CHRM1 (443-458) Antibody, preincubated with the negative control antigen.
      • Anti-CHRM1 (443-458) Antibody
        Expression of Muscarinic acetylcholine receptor M1 in mouse striatum
        Immunohistochemical staining of mouse striatum using Anti-CHRM1 (443-458) Antibody (#AMR-010), (1:100). M1 mAChR staining (red) appears in the matrix (M), not in the patches (P). Blood vessels in the matrix (arrow) are also stained. DAPI is used as the counterstain (blue).
    References
    • 1. Felder, C.C. et al. (2000) J. Med. Chem. 43, 4333.
    • 2. Forsythe, S.M. et al. (2002) Am. J. Respir. Cell. Mol. Biol. 26, 298.
    • 3. Ferreira, A.R. et al. (2003) Pharmacol. Biochem. Behav. 74, 411.
    • 4. Van der Zee, E.A. et al. (1999) Prog. Neurol. 58, 409.
    • 5. Levey, A.I. et al. (1991) J. Neurosci. 11, 3218.
    • 6. Wang, J. et al. (2000) Am. J. Physiol. Gastrointest. Liver Physiol. 279, G1059.
    • 7. Budd, S.C. et al. (2003) J. Biol. Chem. 278, 19565.
      • The action of the neurotransmitter acetylcholine (ACh) is mediated through two types of receptors, the ionotrophic nicotinic receptors and the metabotrophic muscarinic receptors. The muscarinic receptors  belong to the superfamily of 7-TM G-protein-coupled receptors. Five subtypes of muscarinic receptors have been cloned and named M1-M5.1-2

        The muscarinic receptors are widely distributed throughout the body, but are predominantly expressed within the parasympathetic nervous system and exert both excitatory and inhibitory control over central and peripheral tissues.1-2

        Muscarinic receptors participate in a number of physiological functions such as regulation of heart rate, muscle contraction, cognition, sensory processing and motor control.1 They also participate in learning and memory processing.3-4 The M1 receptors are the most abundant muscarinic subtype in the cortex and striatum. M1 receptors were also localized in the myenteric plexus where they function as autoreceptors to enhance the release of ACh from the nerves.5-6

        The M1, M3 and M5 receptors, which are coupled to Gq/11 proteins, can protect cells from undergoing apoptosis induced by DNA damage. The signaling mechanism that mediates this anti-apoptotic response is still poorly understood. However, it was recently reported that a poly-basic motif in the C-terminus tail of the M1, M3 and M5 receptors is an essential element for the anti-apoptotic response of those receptors.7

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
    IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 11/11/2019

    Anti-CHRM1 (443-458) Antibody (#AMR-010) is a highly specific antibody directed against an epitope of the human M1 muscarinic receptor. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize M1 receptor from mouse, rat, and human samples.

    For research purposes only, not for human use
    Citations
    Related Products
        • Anti-CHRM1 Antibody (#AMR-001).
          This antibody recognizes a different epitope and thus can also be used as a control for detecting M1 receptor.