Anti-NIPA2 (extracellular) Antibody

Magnesium Transporter NIPA2, Non-imprinted in Prader-Willi/Angelman syndrome region protein 2 homolog
    Cat #: ANT-142
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)ETLNEMSHKLGD, corresponding to amino acid residues 137-148 of mouse magnesium transporter NIPA2 (Accession Q9JJC8). 2nd extracellular loop.
    Accession (Uniprot) Number Q9JJC8
    Gene ID 93790
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, human – identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, lci, wb
    May also work in: ifc, ih, ip
    Western blot
    Western blot analysis of rat (lanes 1 and 3) and mouse heart (lanes 2 and 4) lysates:
    1,2. Anti-NIPA2 (extracellular) Antibody (#ANT-142), (1:200).
    3,4. Anti-NIPA2 (extracellular) Antibody preincubated with the control peptide antigen.
    Live cell imaging / Immunocytochemistry
    Expression of NIPA2 in rat C6 cells
    Immunocytochemical staining of rat brain glioma C6 cells. A. Extracellular staining of cells with Anti-NIPA2 (extracellular) Antibody (#ANT-142), (1:50), (red). B. Merged image of A with live view of cell.
    References
    1. Goeckeler, Z.M. et al. (2008) Am. J. Physiol. Cell. Physiol. 295, C994.
    2. Jiang, Y. et al. (2012) Hum. Genet. 131, 1217.
    Scientific background

    The NIPA family is currently comprised of 4 members: NIPA1-4. These members have a molecular similarity of 40%. They are integral membrane proteins which function as Mg2+ transporters.

    While NIPA2 is highly selective for Mg2+ transportation, other members of the family transport other cations as well. NIPA2 has 9 transmembrane protein domains. It is also found in the early endosome and transfers extracellular Mg2+ into the cytoplasm. NIPA2 is highly conserved among vertebrates; human and mouse NIPA2 have a 96% amino acid identity. NIPA2 is expressed in the heart, CNS and pancreas. Its expression is upregulated in MDCT cells cultured in low-magnesium levels compared to normal magnesium.

    Several imprinted genes have been identified as candidates for prader-willi syndrome in the 15q11-q13 region however the involvement of NIPA2, which resides in this region, remains unclear. Currently there is no evidence that the gene is directly involved in the many complexities of the syndrome- Mouse models of prader-willi-like pathologies include severe failure to thrive and early postnatal lethality have not implicated NIPA21.

    NIPA2 has also been recently implicated in the pathogenesis of childhood absence epilepsy (CAE). NIPA2 mutations have been found in some CAE patients. These mutations comprise of 2 missense mutations and a small insertion mutation. All mutations are heterozygous and are inherited from the paternal side. These mutations affect the highly conserved amino-acid structure of the transporter2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 29/10/2018

    Alomone Labs is pleased to offer an antibody directed against an epitope of mouse magnesium transporter NIPA2. Anti-NIPA2 (extracellular) Antibody (#ANT-142) can be used in western blot and immunocytochemistry in living cells. It has been designed to recognize NIPA2 from rat, human and mouse samples.

    For research purposes only, not for human use
    Citations
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