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Anti-NIPAL4 (extracellular) Antibody

Magnesium transporter NIPA4, NIPA-like protein 4, NIPA-like domain containing 4, Ichthyin, ICHYN, ICHN, Non-imprinted in Prader-Willi/Angelman syndrome region protein 4

Cat #: ANT-144
Alternative Name Magnesium transporter NIPA4, NIPA-like protein 4, NIPA-like domain containing 4, Ichthyin, ICHYN, ICHN, Non-imprinted in Prader-Willi/Angelman syndrome region protein 4
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EKVTTIMEMASKMKD, corresponding to amino acid residues 242-256 of human magnesium transporter NIPA4 (Accession Q0D2K0). 2nd extracellular loop.
Accession (Uniprot) Number Q0D2K0
Gene ID 348938
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 13/15 amino acid residues identical; rat - 12/15 amino acid residues identical.
RRID AB_2756732.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*, lci*
Western blot
  • Western blot analysis of human acute monocytic leukemia cell line THP-1 (lanes 1 and 2), mouse B cell lymphoma cell line WEHI-231 (lanes 3 and 4), rat brain membranes (lanes 5 and 7) and mouse brain membranes (lanes 6 and 8):
    Western blot analysis of human acute monocytic leukemia cell line THP-1 (lanes 1 and 2), mouse B cell lymphoma cell line WEHI-231 (lanes 3 and 4), rat brain membranes (lanes 5 and 7) and mouse brain membranes (lanes 6 and 8):
    1,3,5,7. Anti-NIPAL4 (extracellular) Antibody (#ANT-144), (1:400).
    2,4,6,8. Anti-NIPAL4 (extracellular) Antibody, preincubated with NIPAL4 (extracellular) Blocking Peptide (#BLP-NT144).
Indirect flow cytometry
  • Cell surface detection of Magnesium transporter NIPA4 in THP-1 (human acute monocytic leukemia cell line) living cells:
    Cell surface detection of Magnesium transporter NIPA4 in THP-1 (human acute monocytic leukemia cell line) living cells:
    ___ Unstained cells.
    ___ Cells stained with Anti-NIPAL4 (extracellular) Antibody (#ANT-144), (5 µg/5x105 cells).
References
  1. Quamme, G.A. (2010) Am. J. Physiol. Cell Physiol. 298, C407.
Scientific background

The NIPA family is currently comprised of 4 members- NIPA1-4. These members have a molecular similarity of 40%. NIPA proteins are integral membrane proteins which function as Mg2+ transporters.

The NIPA4 gene in mice is located on chromosome 11B1.1 and the human corresponding gene, hIchthyin is located on chromosome 5q33.3. Both genes share an 85% amino acid similarity. Interestingly, NIPA2-4 show greater common identity in their ancestral invertebrate polypeptides than they do to NIPA1 suggesting different physiological roles. NIPA4 mediates Mg2+ uptake with a Km of 0.36 mM but unlike NIPA2, which is selective for Mg2+, it has been found to transport other cations as well.

Mutations in the hIchthyin gene provide the basis of one form of autosomal-recessive congenital ichthyosis (ARCI). ARCI is a heterogenous group of disorders with abnormal differentiation and desquamation of the epidermis, leading to widespread scaling and erythema of the skin. To this day six homozygous mutations have been found, including one nonsense and five missense mutations, in a large number of consanguineous families presenting with ichthyosis. The function of ichthyin in skin differentiation is not fully understood but it has been suggested that because lipid dysfunctions are involved with the other ARCI disorders ichthyin might perform as a membrane receptor for trioxilins of the hepoxilin pathway.

Although some NIPA proteins have been implicated as candidates for prader-willi syndrome their involvement remains unclear. Currently there is no evidence that NIPA4 is directly involved in the many complexities of the syndrome1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human magnesium transporter NIPA4. Anti-NIPAL4 (extracellular) Antibody (#ANT-144) can be used in western blot and indirect flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the transporter in living cells. It has been designed to recognize NIPAL4 from mouse, rat and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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