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Anti-MC1 Receptor Antibody

Melanocortin receptor 1, MC1R, MC1-R, Melanocyte-stimulating hormone receptor, MSHR, MSH-R, SHEP2

Cat #: AMR-020
Alternative Name Melanocortin receptor 1, MC1R, MC1-R, Melanocyte-stimulating hormone receptor, MSHR, MSH-R, SHEP2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, r
Immunogen
  • Peptide (C)HAQGIARLHKRQRPVH, corresponding to amino acid residues 217-232 of human MC1R (Accession Q01726). 3rd intracellular loop.
Accession (Uniprot) Number Q01726
Gene ID 4157
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - 11/6 amino acids residues identical.
RRID AB_2039974.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Probably won’t recognize mouse.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.6 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of human normal skin fibroblast cell line Malme-3 (lanes 1 and 4) and human malignant melanoma cell lines Malme-3M (lanes 2 and 5) and A875 (lanes 3 and 6):
    Western blot analysis of human normal skin fibroblast cell line Malme-3 (lanes 1 and 4) and human malignant melanoma cell lines Malme-3M (lanes 2 and 5) and A875 (lanes 3 and 6):
    1,2,3. Anti-MC1 Receptor Antibody (#AMR-020), (1:500).
    4,5,6. Anti-MC1 Receptor Antibody, preincubated with MC1 Receptor Blocking Peptide (#BLP-MR020).
  • Western blot analysis of rat adrenal lysate:
    Western blot analysis of rat adrenal lysate:
    1. Anti-MC1 Receptor Antibody (#AMR-020), (1:400).
    2. Anti-MC1 Receptor Antibody, preincubated with MC1 Receptor Blocking Peptide (#BLP-MR020).
Immunohistochemistry
  • Expression of MC1R in normal skin and melanoma
    Expression of MC1R in normal skin and melanoma
    Immunohistochemical staining of paraffin embedded normal skin and melanoma sections using Anti-MC1 Receptor Antibody (#AMR-020) (1:100). MCR1 staining (red-brown color is highly specific in A. epidermal cells, B. eccrine sweat gland cells and C. melanoma cells. Color reaction was obtained with DAB. Hematoxilin is used as the counterstain.
Immunocytochemistry
  • Expression of MC1R in human Malme-3M cells
    Expression of MC1R in human Malme-3M cells
    Immunocytochemical staining of human paraformaldehyde fixed and permeabilized malignant melanoma cell lines (Malme-3M). A. Cells were stained with Anti-MC1 Receptor Antibody (#AMR-020), (1:200) followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody. B. Live view of the same field as in (A). C. Computer merge of (A) and (B).
References
  1. Abdel-Malek, Z.A. (2001) Cell. Mol. Life Sci. 58, 434.
  2. Gantz, I. and Fong, T.M. (2003) Am. J. Physiol. 284, E468.
  3. Wikberg, J.E. et al. (2000) Pharm. Res. 42, 393.
  4. Dinulescu, D.M. and Cone, R.D. (2000) J. Biol. Chem. 275, 6695.
  5. Kanetsky, P.A. et al. (2006) Cancer Res. 66, 9330.
  6. Catania, A. et al. (2004) Pharmacol. Rev. 56, 1.
Scientific background

Melanocortin receptor 1 (MC1R) belongs to a five-member receptor family known as the melanocortin receptors. The melanocortin receptors are members of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.

The receptors ligands, the melanocortins, are a group of structurally derived peptides consisting of α-, β- and γ-melanocyte stimulating hormone (α, β, γ-MSH) and the adrenocorticotropic hormone (ACTH) all of which are derived from the post-translational processing of a common precursor peptide, proopiomelanocortin (POMC).1,2,3

One of the most salient features of the melanocortin signaling system is the presence of two endogenous antagonists, that is proteins that bind specifically to the receptor but instead of activating it have an inhibitory effect. The antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP).4

All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti and AGRP) with different affinities.

MC1R was the first member of the melanocortin receptor family to be cloned. The receptor transduces signals via Gs resulting in the activation of adenylate cyclase and production of cAMP. 

MC1R binds its ligands with the following potency: α-MSH = ACTH > β-MSH > γ-MSH. MC1R also binds the endogenous antagonist agouti with high affinity.1, 2

MC1R can be described as the “classical” melanocyte α-MSH receptor. The receptor is expressed in the skin where it has a key role in determining skin and hair pigmentation. In fur-bearing mammals, the local ratio between α-MSH and agouti will determine coat pigmentation as α-MSH stimulates and agouti inhibits melanin production.

In humans, especially in Caucasians, the MC1R gene is highly polymorphic and several allelic variants have been correlated with red-hair, poor tanning ability and increased risk of melanoma.1,3,5 In addition, MC1R expression in melanoma has been shown to be upregulated up to 20-fold when compared to normal melanocytes.

The melanocortins and particularly α-MSH have significant anti-inflammatory properties. Since α-MSH binds to MC1R with the highest potency, it was proposed that the latter mediated the anti-inflammatory effects of α-MSH. Indeed, MC1R expression has been demonstrated in several cells of the immune system including macrophages and neutrophils.6

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title: Anti-MC1 Receptor Antibody
Expression of MC1R in human kidney.Immunohistochemical staining of human kidney biopsy sections using Anti-MC1 Receptor Antibody (#AMR-020). A. MC1R staining (green) is detected in the glomerular and immune-colocalizes with synaptopodin, a podocyte marker. B. Enlarged images of glomerular capillaries. C. MC1R staining does not colocalize with endothelial-specific lectin Ulex europaeus agglutinin I, an endothelial cell marker. D. Primary antibody was omitted.Adapted from Lindskog, A. et al. (2010) J. Am. Soc. Nephrol. 21, 1290. with permission of the American Society of Nephrology.
Last update: 11/04/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human melanocortin receptor 1. Anti-MC1 Receptor Antibody (#AMR-020) can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize MC1R from human and rat samples. The antibody is unlikely to recognize MC1R from mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Rat liver lysate (1:500).
    Lonati, C. et al. (2014) Peptides 50, 145.
  2. Rat kidney and testis.
    Si, J. et al. (2013) Kidney Int. 83, 635.
  3. Human kidney lysate (1:500).
    Lindskog, A. et al. (2010) J. Am. Soc. Nephrol. 21, 1290.
Immunohistochemistry citations
  1. Rat kidney sections.
    Si, J. et al. (2013) Kidney Int. 83, 635.
  2. Human kidney sections (1:100).
    Lindskog, A. et al. (2010) J. Am. Soc. Nephrol. 21, 1290.
Immunocytochemistry citations
  1. Rat renal tubular epithelial cells.
    Si, J. et al. (2013) Kidney Int. 83, 635.
More product citations
  1. Shen, Y. et al. (2013) J. Neurosci. 33, 464.
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