- Peptide (C)GDHNFMRREIKIEGD, corresponding to amino acids 24-38 of rat mGluR3 (Accession P31422). Extracellular, N-terminus.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-mGluR3 (extracellular) Antibody (#AGC-012), (1:600).
3,4. Anti-mGluR3 (extracellular) Antibody, preincubated with mGluR3 (extracellular) Blocking Peptide (#BLP-GC012).
- Expression of mGluR3 in rat brain stemImmunohistochemical staining of frozen brain stem sections using Anti-mGluR3 (extracellular) Antibody (#AGC-012), (1:100) followed by goat anti-rabbit AlexaFluor-555 secondary antibody (red). Staining is present in neuronal cell bodies (arrows) in the brainstem nuclei. Hoechst 33342 is used as the counterstain (blue).
- Expression of mGluR3 in rat PC12 cellsCell surface detection of mGluR3 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-mGluR3 (extracellular) Antibody (#AGC-012), (1:50), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.
L-glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptor family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.
Group II of the metabotropic glutamate receptors includes the mGluR2 and mGluR3 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus that contains the glutamate binding site, and an intracellular C-terminus.1,2 mGluR2 and mGluR3 are coupled to Gi/G0 and hence inhibit cAMP formation following receptor activation.1,2
mGluR3 is expressed in neurons and glia in many brain regions including the cerebral cortex, hippocampus and brain stem. mGluR3 expression is predominantly presynaptic consistent with its role as an inhibitory autoreceptor, that is, activation of mGluR3 by glutamate inhibits additional glutamate release from the same neurons.1,2
Several lines of evidence suggest that mGluR3 is important for long term depression, glial function and neuroprotection. Some studies, though not all, have shown a genetic association of mGluR3 gene polymorphisms with psychosis and with schizophrenia-related phenotypes. Based in these findings, mGluR3 has been suggested as a therapeutic target for both psychosis and schizophrenia.3
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat mGluR3. The epitope is specific for mGluR3 and will not cross-react with the closely related mGluR2. Anti-mGluR3 (extracellular) Antibody (#AGC-012) can be used in western blot, live cell imaging and immunohistochemistry applications. It has been designed to recognize mGluR3 from rat and mouse samples.
- Anti-mGluR1 (extracellular) Antibody (#AGC-006)
- Anti-mGluR2 (extracellular) Antibody (#AGC-011)
- Anti-mGluR4 (extracellular) Antibody (#AGC-014)
- Anti-mGluR5 (extracellular) Antibody (#AGC-007)
- Anti-mGluR6 (extracellular) Antibody (#AGC-026)
- Anti-mGluR7 (extracellular) Antibody (#AGC-017)
- Anti-mGluR8 (extracellular) Antibody (#AGC-028)
- Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001)
- Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody (#AGC-003-AR)
- Anti-GluR2 (GluA2) (extracellular)-ATTO Fluor-488 Antibody (#AGC-005-AG)
- Anti-GRIK2 (GluK2) Antibody (#AGC-009)