Anti-mGluR5 (extracellular)-ATTO-594 Antibody

Metabotropic glutamate receptor 5, GRM5, mGlu5, GPRC1E
    Cat #: AGC-007-AR
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
    Immunogen
    Peptide EGFAQENSKYNKTC, corresponding to amino acids 367-380 of rat mGluR5 (Accession P31424). Extracellular, N-terminus.
    Accession (Uniprot) Number P31424
    Gene ID 24418
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 13/14 amino residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGC-007), and immunohistochemistry (labeled antibody).
    Applications: ih
    May also work in: ic, lci
    Immunohistochemistry
    Expression of mGluR5 in rat hippocampus
    Immunohistochemical staining of perfusion-fixed frozen rat hippocampus sections using Anti-mGluR5 (extracellular)-ATTO-594 Antibody (#AGC-007-AR), (1:50). A. mGluR5 (red) is detected in pyramidal layer neurons (arrow). B. DAPI is used to visualize cell nuclei (blue). C. Merge of A and B.
    Immuno-colocalization of NK1 and mGluR5 in rat DRG
    Immunohistochemical staining of rat dorsal root ganglion using Anti-Neurokinin Receptor 1 (NK1) (extracellular)-ATTO-488 Antibody (#ATR-001-AG), (green), (1:60) and Anti-mGluR5 (extracellular)-ATTO-594 Antibody (#AGC-007-AR), (red), (1:60).  A. NK1 staining. B. mGluR5 staining appears in both cells (horizontal arrow) and fibers (vertical arrow). C. Merge of A and B demonstrates the co-localization of NK1 and mGluR5 receptors. Nuclei staining using DAPI as the counterstain (blue).
    Immuno-colocalization of TRPV1 and mGluR5 in rat DRG
    Immunohistochemical staining of rat dorsal root ganglion using Anti-Rat TRPV1 (VR1) (extracellular)-ATTO-488 Antibody (#ACC-029-AG), (1:60) and Anti-mGluR5 (extracellular)-ATTO-594 Antibody (#AGC-007-AR), (1:60). A. TRPV1 staining (green). B. mGluR5 staining of the same section (red). C. Merge of A and B demonstrates co-localization of TRPV1 and mGluR5 in DRG cells. Nuclei were stained using DAPI as the counterstain (blue).
    References
    1. Kew, J.N.C. and Kemp, J.A. (2005) Psychopharmacology (Berl). 179, 4.
    2. Ferraguti, F. and Shigemoto, R. (2006) Cell Tissue Res326, 428.
    3. Dhami, G.K. and Ferguson, S.S. (2006) Pharmacol. Ther. 111, 260.
    Scientific background

    L-Glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptors family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.

    Group I of the metabotropic glutamate receptors includes the mGluR1 and mGluR5 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus domain and an intracellular C-terminus one. The N-terminus domain of group I receptors contains the glutamate binding site while the cytoplasmic C-terminus domain has an important role in the regulation of receptor activity through interactions with other proteins such as the Homer adaptor proteins.

    mGluR1 and mGluR5 receptors signal through Gq/G11 that activates phospholipase C and ultimately produces an increase in inositol trisphosphate and cytosolic Ca2+. More downstream signaling pathways include activation of PKC and modulation of Ca2+ and K+ ion channels. Activation of signaling pathways independent of G-proteins has also been reported.

    mGluR5 is predominantly expressed in nervous tissue although expression in several non-neural cell types has also been described. In the brain it is highly expressed in the cortex, basal ganglia and hippocampus.

    The mGluR5 receptor is involved in several physiological processes such as neuronal development, induction of long-term potentiation (LTP) and depression (LTD) as well as in pathological disorders such as brain trauma, chronic pain, Parkinson’s and Huntington’s disease.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-mGluR5 (extracellular)-ATTO-594 AntibodyImmuno-colocalization of TRPV2 and mGluR5 in rat DRGImmunohistochemistry of rat dorsal root ganglion using Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody (#AGP-033) (1:60) and Anti-mGluR5 (extracellular)-ATTO-594 Antibody (#AGC-007-AR) (1:60). A. TRPV2 staining (green). B. mGluR5 staining (red). C. Merge of A and B demonstrates co-localization of TRPV2 and mGluR5 in DRG cells. Nuclei are stained using DAPI as the counterstain (blue).

    Last update: 06/12/2018

    Anti-mGluR5 (extracellular) Antibody (#AGC-007) is a highly specific antibody directed against an extracelluar epitope of rat mGluR5. The antibody can be used in western blot analysis, immunhistochemical and live cell imaging applications. It has been designed to recognize mGluR5 from rat, mouse and human samples.

    Anti-mGluR5 (extracellular)-ATTO-594 Antibody (#AGC-007-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-mGluR5 (extracellular)-ATTO-594 Antibody has been tested in immunohistochemistry and is specially suited to experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use