Overview
Type: Synthetic peptide
Form: Lyophilized powder
mGluR5 (extracellular) Blocking Peptide (#BLP-GC007) is the original antigen used for immunization during Anti-mGluR5 (extracellular) Antibody (#AGC-007) generation. The blocking peptide binds and ‘blocks’ Anti-mGluR5 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-mGluR5 (extracellular) Antibody (#AGC-007), (1:500).
2. Anti-mGluR5 (extracellular) Antibody, preincubated with mGluR5 (extracellular) Blocking Peptide (#BLP-GC007).
Expression of mGluR5 in rat cerebellumImmunohistochemical staining of perfusion-fixed frozen rat cerebellum sections using Anti-mGluR5 (extracellular) Antibody (#AGC-007), (1:50). mGluR5 (red) was detected in cerebellar Purkinje cells (vertical arrows) and in the molecular layer (horizontal arrows). Staining with mouse anti-parvalbumin (green) revealed co-localization in Purkinje but not in the molecular layer. Little staining of mGluR5 was detected in the granule layer. DAPI counterstain is used to visualize nuclei of all cells (blue).
Expression of mGluR5 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat hippocampus sections using Anti-mGluR5 (extracellular) Antibody (#AGC-007), (1:50). mGluR5 (red) was detected in CA3 cells (arrows). Staining with mouse anti-parvalbumin (green) revealed co-localization in pyramidal layer. DAPI counterstain was used to visualize nuclei of all cells (blue).
Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:1,2. Guinea pig Anti-mGluR5 (extracellular) Antibody (#AGC-007-GP), (1:400).
3,4. Guinea pig Anti-mGluR5 (extracellular) Antibody, preincubated with mGluR5 (extracellular) Blocking Peptide (#BLP-GC007).
Following a broad screen of secondary antibodies, the following were used for this application:
#106-035-006 (Jackson ImmunoResearch)
#A7289 (Sigma)
Properties
Sequence
- EGFAQENSKYNKTC, corresponding to amino acids 367-380 of rat mGluR5 (Accession P31424).
Accession (Uniprot) Number P31424
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
