Overview
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-mGluR5 (extracellular) Antibody (#AGC-007), Anti-mGluR5 (extracellular)-ATTO Fluor-594 Antibody (#AGC-007-AR) and Guinea pig Anti-mGluR5 (extracellular) Antibody (#AGP-090). Intended to be used as a negative control.
Applications: bl
For research purposes only, not for human use
Applications
Applications
Blocking
Western blot analysis of rat brain membranes:1. Anti-mGluR5 (extracellular) Antibody (#AGC-007), (1:500).
2. Anti-mGluR5 (extracellular) Antibody, preincubated with mGluR5 (extracellular) Blocking Peptide (#BLP-GC007).
Expression of mGluR5 in rat cerebellumImmunohistochemical staining of perfusion-fixed frozen rat cerebellum sections using Anti-mGluR5 (extracellular) Antibody (#AGC-007), (1:50). mGluR5 (red) was detected in cerebellar Purkinje cells (vertical arrows) and in the molecular layer (horizontal arrows). Staining with mouse anti-parvalbumin (green) revealed co-localization in Purkinje but not in the molecular layer. Little staining of mGluR5 was detected in the granule layer. DAPI counterstain is used to visualize nuclei of all cells (blue).
Expression of mGluR5 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat hippocampus sections using Anti-mGluR5 (extracellular) Antibody (#AGC-007), (1:50). mGluR5 (red) was detected in CA3 cells (arrows). Staining with mouse anti-parvalbumin (green) revealed co-localization in pyramidal layer. DAPI counterstain was used to visualize nuclei of all cells (blue).
Expression of mGluR5 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat hippocampus sections using Anti-mGluR5 (extracellular)-ATTO Fluor-594 Antibody (#AGC-007-AR), (1:50). A. mGluR5 (red) is detected in pyramidal layer neurons (arrow). B. DAPI is used to visualize cell nuclei (blue). C. Merge of A and B.
Multiplex staining of NK1 Receptor and mGluR5 in rat DRGImmunohistochemical staining of rat dorsal root ganglion using Anti-Neurokinin 1 Receptor (NK1R) (extracellular)-ATTO Fluor-488 Antibody (#ATR-001-AG), (green), (1:60) and Anti-mGluR5 (extracellular)-ATTO Fluor-594 Antibody (#AGC-007-AR), (red), (1:60). A. NK1 receptor staining. B. mGluR5 staining appears in both cells (horizontal arrow) and fibers (vertical arrow). C. Merge of A and B demonstrates the co-localization of NK1 and mGluR5 receptors. Nuclei staining using DAPI as the counterstain (blue).
Multiplex staining of TRPV1 and mGluR5 in rat DRGImmunohistochemical staining of rat dorsal root ganglion using Anti-Rat TRPV1 (VR1) (extracellular)-ATTO Fluor-488 Antibody (#ACC-029-AG), (1:60) and Anti-mGluR5 (extracellular)-ATTO Fluor-594 Antibody (#AGC-007-AR), (1:60). A. TRPV1 staining (green). B. mGluR5 staining of the same section (red). C. Merge of A and B demonstrates co-localization of TRPV1 and mGluR5 in DRG cells. Nuclei were stained using DAPI as the counterstain (blue).
Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:1,2. Guinea pig Anti-mGluR5 (extracellular) Antibody (#AGP-090), (1:400).
3,4. Guinea pig Anti-mGluR5 (extracellular) Antibody, preincubated with mGluR5 (extracellular) Blocking Peptide (#BLP-GC007).
Following a broad screen of secondary antibodies, the following were used for this application:
#106-035-006 (Jackson ImmunoResearch)
#A7289 (Sigma)
Properties
Properties
Sequence
- EGFAQENSKYNKTC, corresponding to amino acids 367-380 of rat mGluR5 (Accession P31424).
Accession (Uniprot) Number P31424
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Shipping and Ordering information
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