Overview
Cat #: BLP-GC007
Type: Synthetic peptide
Form: Lyophilized powder
mGluR5 (extracellular) Blocking Peptide (#BLP-GC007) is the original antigen used for immunization during Anti-mGluR5 (extracellular) Antibody (#AGC-007) generation. The blocking peptide binds and ‘blocks’ Anti-mGluR5 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB-western blot, IHC-Immunohistochemistry
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain membranes:1. Anti-mGluR5 (extracellular) Antibody (#AGC-007), (1:500).
2. Anti-mGluR5 (extracellular) Antibody, preincubated with mGluR5 (extracellular) Blocking Peptide (#BLP-GC007). - Expression of mGluR5 in rat cerebellumImmunohistochemical staining of perfusion-fixed frozen rat cerebellum sections using Anti-mGluR5 (extracellular) Antibody (#AGC-007), (1:50). mGluR5 (red) was detected in cerebellar Purkinje cells (vertical arrows) and in the molecular layer (horizontal arrows). Staining with mouse anti-parvalbumin (green) revealed co-localization in Purkinje but not in the molecular layer. Little staining of mGluR5 was detected in the granule layer. DAPI counterstain is used to visualize nuclei of all cells (blue).
- Expression of mGluR5 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat hippocampus sections using Anti-mGluR5 (extracellular) Antibody (#AGC-007), (1:50). mGluR5 (red) was detected in CA3 cells (arrows). Staining with mouse anti-parvalbumin (green) revealed co-localization in pyramidal layer. DAPI counterstain was used to visualize nuclei of all cells (blue).
- Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:1,2. Guinea pig Anti-mGluR5 (extracellular) Antibody (#AGC-007-GP), (1:400).
3,4. Guinea pig Anti-mGluR5 (extracellular) Antibody, preincubated with mGluR5 (extracellular) Blocking Peptide (#BLP-GC007).
Following a broad screen of secondary antibodies, the following were used for this application:
#106-035-006 (Jackson ImmunoResearch)
#A7289 (Sigma)
Properties
Accession (Uniprot) Number P31424
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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