- Peptide CIEQHQLNKERELIER, corresponding to amino acid residues 191-206 of human mitochondrial calcium uniporter (Accession Q8NE86). N-terminus, mitochondrial matrix.
- Western blot analysis of rat kidney (lanes 1 and 5), rat skeletal muscle (lanes 2 and 6), rat brain (lanes 3 and 7) and mouse brain (lanes 4 and 8) lysates:1-4. Anti-MCU Antibody (#ACC-328), (1:200).
5-8. Anti-MCU Antibody, preincubated with MCU Blocking Peptide (#BLP-CC328).
- Expression of MCU in mouse midbrain.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-MCU Antibody (#ACC-328), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse substantia nigra region, showed immunoreactivity (green) in neuronal profiles in the substantia nigra pars compacta (SNC, horizontal arrows). B. Pre-incubation of the antibody with MCU Blocking Peptide (BLP-CC328), suppressed staining. Cell nuclei are stained with DAPI (blue). SNR = substantia nigra pars reticulata.
Mitochondrial calcium uniporter (MCU) is a 480 kDa mitochondrial inner membrane protein, a multimer that forms a calcium (Ca2+) channel in the lipid bilayer. MCU is one of the most important and highly selective channels responsible for mitochondrial calcium uptake. This uptake plays an important role in cell energy balance and in cell fate determination.
The MCU protein is comprised of Mcu which functions as the pore-forming and calcium-conducting subunit, encoded by the Ccdc109a gene. The other units- MICU1 (calcium uptake protein 1, mitochondrial), MICU2 (calcium uptake protein 2, mitochondrial), EMRE (essential MCU regulator, mitochondrial), MCUR1 (mitochondrial calcium uniporter regulator 1) and MCUb (calcium uniporter regulatory subunit MCUb, mitochondrial) all function as regulatory subunits1-3.
Studies have demonstrated that knockdown of Mcu uniport in isolated cardiomyocytes enhances peak levels in cytosolic Ca2+ oscillation, resulting in an increase of muscle contraction2.
Mcu pore-forming subunit is a homo-oligomer with two coil-coiled domains and two transmembrane domains (TM) separated by a short hydrophilic loop enriched in acidic amino acids. The second transmembrane helices from five Mcu subunits form a hydrophilic pore in the inner membrane of the mitochondria. The DXXE motif forms the pore entrance in the inter-membrane space. The N- and the C-terminus of MCU face the matrix1,3.
Species reactivity key:
Anti-MCU Antibody (#ACC-328) is a highly selective antibody directed against an epitope of the human mitochondrial calcium uniporter. The antibody can be used in western blot analysis. It has been designed to recognize MCU from human, mouse, and rat samples.