Anti-Mitochondrial Calcium Uniporter

MCU, Ccdc109a, HsMCU, Coiled-coil domain-containing protein 109A
  • New
Cat #: ACC-328
Sizes: 25 µl, 50 µl, 0.2 ml
  • Lyophilized Powder
  • Antigen Incl.
  • QC Tested
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide CIEQHQLNKERELIER, corresponding to amino acid residues 191-206 of human Mitochondrial Calcium Uniporter (Accession Q8NE86). N-terminus, mitochondrial matrix.
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse – 15/16 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
    Western blot
    Western blot analysis of rat kidney (lanes 1 and 5), rat skeletal muscle (lanes 2 and 6), rat brain (lanes 3 and 7) and mouse brain (lanes 4 and 8) lysates:
    1-4. Anti-Mitochondrial Calcium Uniporter antibody (#ACC-328), (1:200).
    5-8. Anti-Mitochondrial Calcium Uniporter antibody preincubated with the control peptide antigen.
    References
    1. Mammucari, C. et al. (2017) Front. Oncol. 7, 139.
    2. Kon, N. et al. (2017) Cell Death Discov. 3, 17045.
    3. Liao, Y. et al. (2017) Int. J. Mol. Sci. 18, 2.
    Scientific background

    Mitochondrial Calcium Uniporter (MCU) is a 480 kDa mitochondrial inner membrane protein, a multimer that forms a calcium (Ca2+) channel in the lipid bilayer. MCU is one of the most important and highly selective channels responsible for mitochondrial calcium uptake. This uptake plays an important role in cell energy balance and in cell fate determination.

    The MCU protein is comprised of Mcu which functions as the pore-forming and calcium-conducting subunit, encoded by the Ccdc109a gene. The other units- MICU1 (calcium uptake protein 1, mitochondrial), MICU2 (calcium uptake protein 2, mitochondrial), EMRE (essential MCU regulator, mitochondrial), MCUR1 (mitochondrial calcium uniporter regulator 1) and MCUb (calcium uniporter regulatory subunit MCUb, mitochondrial) all function as regulatory subunits1-3.

    Studies have demonstrated that knockdown of Mcu uniport in isolated cardiomyocytes enhances peak levels in cytosolic Ca2+ oscillation, resulting in an increase of muscle contraction2.

    Mcu pore-forming subunit is a homo-oligomer with two coil-coiled domains and two transmembrane domains (TM) separated by a short hydrophilic loop enriched in acidic amino acids. The second transmembrane helices from five Mcu subunits form a hydrophilic pore in the inner membrane of the mitochondria. The DXXE motif forms the pore entrance in the inter-membrane space. The N- and the C-terminus of MCU face the matrix1,3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 19/02/2018

    Anti-Mitochondrial Calcium Uniporter (#ACC-328) is a highly selective antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize MCU from human, mouse, and rat samples.

    For research purposes only, not for human use