- Peptide (C)KEMEALSRSKQDD, corresponding to amino acid residues 456-468 of rat MCT2 (Accession Q63344). Intracellular, C-terminus.
- Western blot analysis of mouse testis membranes:1. Anti-MCT2 Antibody (#AMT-012), (1:200).
2. Anti-MCT2 Antibody, preincubated with MCT2 Blocking Peptide (#BLP-MT012).
- Western blot analysis of rat brain lysate (lanes 1 and 3) and rat testis membranes (lanes 2 and 4):1,2. Anti-MCT2 Antibody (#AMT-012), (1:200).
3,4. Anti-MCT2 Antibody, preincubated with MCT2 Blocking Peptide (#BLP-MT012).
- Expression of MCT2 in mouse brain sectionsImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-MCT2 Antibody (#AMT-012), (1:1000). A. MCT2 staining in mouse cortex sections (red) appears in neuronal outlines (arrows). B. MCT2 staining in dorsal pons brain stem regions (red) appears in neuronal outlines (horizontal arrows) and in fibers (vertical arrows). 4th V = fourth ventricle. Cell nuclei are stained with DAPI (blue).
The SLC16A family of monocarboxylate transporters (MCTs) is composed of 14 members. MCTs play a central role in cellular metabolism and metabolic communication between tissues. The rapid transport of monocarboxylates such as pyruvate, lactate, and ketone bodies (acetoacetate and β-hydroxybutyrate) across the plasma membrane of cells is facilitated by proton-linked MCTs1. MCTs share similarities in sequence and protein structure, but each member differs from others in the substrates that they transport. For example, lactate among other monocarboxylates, is the primary substrate for MCT1, MCT2, MCT3 and MCT42. All family members are predicted to have 12 transmembrane helices (TMs) with intracellular C- and N-termini as well as a large cytosolic loop3.
MCT2 is distributed in testis, brain, skeletal muscle, heart, and kidney4.
MCTs are important for normal metabolic function and are implicated in disease. Changes in the cerebral density of MCT2 have been reported acutely following hypoxia-ischemia in vivo and in hippocampal slices5.
Species reactivity key:
Anti-MCT2 Antibody (#AMT-012) is a highly specific antibody directed against an epitope of the rat Monocarboxylate transporter 2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MCT2 from rat and mouse samples. The antibody is unlikely to recognize the protein from human samples.