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Anti-MCT2 Antibody

Monocarboxylate transporter 2, Monocarboxylic acid transporter 2, SLC16A7

Cat #: AMT-012
Alternative Name Monocarboxylate transporter 2, Monocarboxylic acid transporter 2, SLC16A7
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)KEMEALSRSKQDD, corresponding to amino acid residues 456-468 of rat MCT2 (Accession Q63344). Intracellular, C-terminus.
Accession (Uniprot) Number Q63344
Gene ID 29735
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 10/13 amino acid residues identical.
RRID AB_2340997.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Not recommended for human samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of mouse testis membranes:
    Western blot analysis of mouse testis membranes:
    1. Anti-MCT2 Antibody (#AMT-012), (1:200).
    2. Anti-MCT2 Antibody, preincubated with MCT2 Blocking Peptide (#BLP-MT012).
  • Western blot analysis of rat brain lysate (lanes 1 and 3) and rat testis membranes (lanes 2 and 4):
    Western blot analysis of rat brain lysate (lanes 1 and 3) and rat testis membranes (lanes 2 and 4):
    1,2. Anti-MCT2 Antibody (#AMT-012), (1:200).
    3,4. Anti-MCT2 Antibody, preincubated with MCT2 Blocking Peptide (#BLP-MT012).
Immunohistochemistry
  • Expression of MCT2 in mouse brain sections
    Expression of MCT2 in mouse brain sections
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-MCT2 Antibody (#AMT-012), (1:1000). A. MCT2 staining in mouse cortex sections (red) appears in neuronal outlines (arrows). B. MCT2 staining in dorsal pons brain stem regions (red) appears in neuronal outlines (horizontal arrows) and in fibers (vertical arrows). 4th V = fourth ventricle. Cell nuclei are stained with DAPI (blue).
References
  1. Lin, R.Y. et al. (1998) J. Biol. Chem. 273, 2895.
  2. Adijanto, J. and Philp, N.J. (2012) Curr. Top. Membr. 70, 275.
  3. Halestrap, A.P. (2012) IUBMB Life 64, 1.
  4. Wang, Q. et al. (2007) Drug Metab. Dispos. 35, 1393.
  5. Prins, M.L. and Giza, C.C. (2006) Dev. Neurosci. 28, 447.
Scientific background

The SLC16A family of monocarboxylate transporters (MCTs) is composed of 14 members. MCTs play a central role in cellular metabolism and metabolic communication between tissues. The rapid transport of monocarboxylates such as pyruvate, lactate, and ketone bodies (acetoacetate and β-hydroxybutyrate) across the plasma membrane of cells is facilitated by proton-linked MCTs1. MCTs share similarities in sequence and protein structure, but each member differs from others in the substrates that they transport. For example, lactate among other monocarboxylates, is the primary substrate for MCT1, MCT2, MCT3 and MCT42. All family members are predicted to have 12 transmembrane helices (TMs) with intracellular C- and N-termini as well as a large cytosolic loop3.

MCT2 is distributed in testis, brain, skeletal muscle, heart, and kidney4.

MCTs are important for normal metabolic function and are implicated in disease. Changes in the cerebral density of MCT2 have been reported acutely following hypoxia-ischemia in vivo and in hippocampal slices5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-MCT2 Antibody (#AMT-012) is a highly specific antibody directed against an epitope of the rat Monocarboxylate transporter 2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MCT2 from rat and mouse samples. The antibody is unlikely to recognize the protein from human samples.

For research purposes only, not for human use
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