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Anti-Na+/H+ Exchanger 5 (NHE-5) Antibody

Sodium/hydrogen exchanger 5, SLC9A5

Cat #: ANX-005
Alternative Name Sodium/hydrogen exchanger 5, SLC9A5
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CAEKELPWKSGQGD, corresponding to amino acid residues 759-772 of rat NHE-5 (Accession Q9Z0X2). Intracellular, C-terminus.
Accession (Uniprot) Number Q9Z0X2
Gene ID 192215
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2756736.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    1,2. Anti-Na+/H+ Exchanger 5 (NHE-5) Antibody (#ANX-005), (1:200).
    3,4. Anti-Na+/H+ Exchanger 5 (NHE-5) Antibody, preincubated with Na+/H+ Exchanger 5/NHE-5 Blocking Peptide (#BLP-NX005).
References
  1. Siyanov, V. and Baltz, J.M. (2013) Biol. Reprod. 88,157.
  2. Ruffin, V.A. et al. (2014) Front. Physiol. 5, 43.
  3. Diering, G.H. et al. (2011) Mol. Biol. Cell 22, 2246.
Scientific background

Intracellular pH regulation in the brain is extremely important under both physiological and physiopathological conditions. Changes in intracellular pH often result in altered neuronal excitability. In mammalian cells, intracellular pH against acidosis is generally regulated by ion transporters located in the plasma membrane called sodium-hydrogen exchangers (Na+/H+ exchanger, NHE)1.
 
Several studies have suggested that Na+/H+ exchange is the predominant pH-regulatory mode in isolated nerve terminals. Na+/Hexchanger also has a role in the regulation of synaptic transmission at glutamatergicGABAergic, and dopaminergic synapses. In addition, NHE transporters also function in a coupled mode to mediate recovery from decreased cell volume1.
 
The NHE family belongs to the Slc9 gene family of Na+ coupled transport proteins and comprises nine mammalian isoforms of Na+/H+ exchangers that are localized to the plasma membrane. NHE1-9 are encoded by the SLC9A1-SLC9A9 genes, respectively.
 
The structure of SLC9 family members includes a short N-terminus and an extensive C-terminus that plays a regulatory role.
 
NHE-5 exchanger corrects intracellular acidosis by exporting H+ in exchange for extracellular Na+, thereby tending to raise intracellular pH. Several studies have suggested that NHE5 plays a critical role in the synaptic pH regulation during the firing of action potentials1.
 
It is predominantly detected in the brain with high expression in multiple regions such as the hippocampus and cerebral cortex3
 
NHE5 knockdown or overexpression of a dominant-negative mutation of NHE5 in cultured hippocampal neurons causes dendritic spine overgrowth. Therefore it has been suggested that NHE5 acts as a negative regulator of activity-dependent spine growth by regulating pH-sensitive synaptic proteins2,3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/06/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat NHE-5. Anti-Na+/H+ Exchanger 5 (NHE-5) Antibody (#ANX-005) can be used in western blot analysis. It has been designed to recognize NHE-5 from human, rat and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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