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Anti-NaV1.8 (SCN10A) (extracellular) Antibody

PN3, SNS, Sodium channel protein type 10 subunit alpha

Cat #: ASC-028
Alternative Name PN3, SNS, Sodium channel protein type 10 subunit alpha
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)EDNYLEEKPRVKSVLE, corresponding to amino acid residues 1173-1188 of rat NaV1.8 (Accession Q62968). 1st extracellular loop, domain III.
Accession (Uniprot) Number Q62968
Gene ID 29571
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human – Not recommended for use with human samples.
RRID AB_2341070.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Not recommended for use with human samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*, lci*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 3) and rat DRG (lanes 2 and 4) lysates:
    Western blot analysis of rat brain (lanes 1 and 3) and rat DRG (lanes 2 and 4) lysates:
    1,2. Anti-NaV1.8 (SCN10A) (extracellular) Antibody (#ASC-028), (1:200).
    3,4. Anti-NaV1.8 (SCN10A) (extracellular) Antibody, preincubated with Nav1.8/SCN10A (extracellular) Blocking Peptide (#BLP-SC028).
References
  1. Wu, L. et al. (2002) NeuroReport 13, 2547.
  2. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27.
  3. Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
  4. Fang, X. et al. (2002) J. Neurosci. 22, 7425.
  5. Fjell, J. et al. (2000) NeuroReport 11, 199.
  6. Renganathan, M. et al. (2003) Brain Res. 959, 235.
Scientific background

Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.9.2-3 The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels.4-5b Expression of the α subunit isoform is developmentally and tissue specific.

Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.8 channel (also called SCN10A, SNS and PN3) is mainly expressed in small-diameter DRG neurons.4-6 TTX-resistant channels have been suggested to play an important role in nociceptive transmission.

Recently, involvement of NaV1.8 in multiple sclerosis (MS) was suggested due to up-regulation of both mRNA and protein in Purkinje cells of MS patients and also in animal models.6

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/08/2022

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat NaV1.8 channel. Anti-NaV1.8 (SCN10A) (extracellular) Antibody (#ASC-028) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting NaV1.8 in living cells. It has been designed to recognize NaV1.8 from rat and mouse samples. The antibody will not recognize NaV1.8 from human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

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