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Anti-Nectin-2/PVRL2 (extracellular) Antibody

Poliovirus receptor-related protein 2, Herpes virus entry mediator B, PRR2, PVRR2, CD112

Cat #: ANR-052
Alternative Name Poliovirus receptor-related protein 2, Herpes virus entry mediator B, PRR2, PVRR2, CD112
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RVIAQPENHAEAQE, corresponding to amino acid residues 148-161 of mouse Nectin-2 (Accession P32507). Extracellular, N-terminus.
Accession (Uniprot) Number P32507
Gene ID 19294
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - identical; human - 10/14 amino acid residues identical.
RRID AB_2756692.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS) pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat spleen lysate (lanes 1 and 4), human Jurkat T cell leukemia cell lysate (lanes 2 and 5) and human K562 erythroleukemia cell lysate (lanes 3 and 6):
    Western blot analysis of rat spleen lysate (lanes 1 and 4), human Jurkat T cell leukemia cell lysate (lanes 2 and 5) and human K562 erythroleukemia cell lysate (lanes 3 and 6):
    1-3. Anti-Nectin-2/PVRL2 (extracellular) Antibody (#ANR-052), (1:200).
    4-6. Anti-Nectin-2/PVRL2 (extracellular) Antibody, preincubated with Nectin-2/PVRL2 (extracellular) Blocking Peptide (#BLP-NR052).
Immunohistochemistry
  • Expression of Nectin-2 in rat hippocampus
    Expression of Nectin-2 in rat hippocampus
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Nectin-2/PVRL2 (extracellular) Antibody (#ANR-052), (1:600), followed by goat-anti-rabbit-AleaxaFluor-488. Nectin-2 staining (green) appears in in apical dendrites of CA1 pyramidal neurons (vertical arrows) and in astrocytes (horizontal arrows). Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of Nectin-2 in live intact human Jurkat T cell leukemia cells:
    Cell surface detection of Nectin-2 in live intact human Jurkat T cell leukemia cells:
    ___ Cells + goat-anti-rabbit-Alexa-488.
    ___ Cells + Anti-Nectin-2/PVRL2 (extracellular) Antibody (#ANR-052), (1:15) + goat-anti-rabbit-Alexa-488.
References
  1. Ogita, H. et al. (2010) Proc. Jpn. Acad. Ser. B Phys. Biol. Sci. 86, 621.
  2. Lopez, M. et al. (1998) Blood 92, 4602.
  3. Rikitake, Y. et al. (2012) J. Cell Sci. 125, 3713.
Scientific background

The Nectin family is known as cell-cell adhesion molecules and comprises four members in this family: nectin-1, nectin-2, nectin-3 and nectin-4 which are encoded by PVRL1, PVRL2, PVRL3, and PVRL4 genes respectively1. They are Ca2+-independent immunoglobulin-like CAMs and are able to trans-interact1.They are localized at adherens junctions between two neighboring cells and binds to afadin through their C-terminal region1.

Nectins, apart from a secreted nectin-1γ, exhibit three immunoglobulin-like loops (one V type and two C2 type) in their extracellular region, a single trans membrane segment and a cytoplasmic tail. Nectins first form a cis-homo-dimer which later on undergoes lateral cluster formation on the cell surface1. Nectins regulate a variety of cellular functions in addition to cell-cell junction formation such as cell polarity, movement, proliferation, differentiation, and survival1.

The PVRL2 gene (which encodes nectin-2) encodes two mRNA isoforms of 3 kb and 4.4 kb both widely expressed in various human tissues including hematopoietic cells of the myelo-monocytic and megakaryocytic lineages and detected also on the surface of endothelial cells2.

Nectin-2 acts as mediator of herpes simplex virus invasion into host cell. A single nucleotide polymorphism in PVRL2 shows an association with later onset of Alzheimers Disease3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse Poliovirus receptor-related protein 2. Anti-Nectin-2/PVRL2 (extracellular) Antibody (#ANR-052) can be used in western blot, immunohistochemistry, and flow cytometry applications. It has been designed to recognize Nectin-2 from mouse, rat and human samples.

For research purposes only, not for human use
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