Anti-Nectin-3/PVRL3 (extracellular) Antibody

Nectin cell adhesion molecule 3, Poliovirus receptor-related 3, CD113
    Cat #: ANR-053
    Alternative Name Nectin cell adhesion molecule 3, Poliovirus receptor-related 3, CD113
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide (C)ETITQISWEKIHGK, corresponding to amino acid residues 84-97 of mouse Nectin-3 (Accession Q9JLB9). Extracellular, N-terminus.
    • Anti-Nectin-3/PVRL3 (extracellular) Antibody
    Accession (Uniprot) Number Q9JLB9
    Gene ID 58998
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human, rat – identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ifc, ih, wb
    May also work in: ic*, ip*, lci*
    Western blot
    • Anti-Nectin-3/PVRL3 (extracellular) Antibody
      Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) testis membranes:
      1,2. Anti-Nectin-3/PVRL3 (extracellular) Antibody (#ANR-053), (1:200).
      3,4. Anti-Nectin-3/PVRL3 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-Nectin-3/PVRL3 (extracellular) Antibody
      Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysate:
      1,2. Anti-Nectin-3/PVRL3 (extracellular) Antibody (#ANR-053), (1:200).
      3,4. Anti-Nectin-3/PVRL3 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-Nectin-3/PVRL3 (extracellular) Antibody
      Western blot analysis of human U-87 MG glioblastoma cell line lysate:
      1. Anti-Nectin-3/PVRL3 (extracellular) Antibody (#ANR-053), (1:200).
      2. Anti-Nectin-3/PVRL3 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-Nectin-3/PVRL3 (extracellular) Antibody
      Expression of Nectin-3 in rat parietal cortex
      Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Nectin-3/PVRL3 (extracellular) Antibody (#ANR-053), (1:100), followed by goat anti-rabbit-AlexaFluor-488. Nectin-3 immunoreactivity (green) appears in cells of the pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).
    Indirect flow cytometry
    • Anti-Nectin-3/PVRL3 (extracellular) Antibody
      Cell surface detection of Nectin-3 in live intact human Jurkat T-cell leukemia cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-Nectin-3/PVRL3 (extracellular) Antibody (#ANR-053), (2.5 µg) + goat-anti-rabbit-FITC.
    1. Rikitake, Y. et al. (2012) J. Cell Sci. 125, 3713.
    2. Ogita, H. et al. (2010) Proc. Jpn. Acad. Ser. B Phys. Biol. Sci. 86, 621.
    Scientific background

    Nectins are Ca2+-independent immunoglobulin-like cell adhesion molecules that localize at adherens junctions between two neighboring cells. They play an important role in mediating asymmetric homotypic and heterotypic cell–cell adhesions. The nectin family contains four members, nectin-1-4, which are encoded by the PVRL1-4 genes, respectively. Nectin-3 is localized mostly in dendrites and partly in axons and is crucial for the specific interactions between axons and dendrites and proper formation of the synapse1,2. Its structure consists of an extracellular region with three immunoglobulin-like loops, one V type and two C2 types.

    Nectins binds afadin at its c-terminal region and connects to the actin cytoskeleton. This interaction plays a major role in the formation of various cell–cell junctions in cooperation with or independently of cadherins cell adhesion molecules1,2.

    Nectins also regulates numerous cellular functions, such as cell movement, proliferation, polarization, survival and differentiation by interacting with various proteins1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-Nectin-3/PVRL3 (extracellular) Antibody (#ANR-053) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize Nectin-3 from human, rat, and mouse samples.

    For research purposes only, not for human use



    Scientific Background


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