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Anti-Netrin-3 Antibody

NTN3, Netrin-2-Like Protein, NTN2L

Cat #: ANR-123
Alternative Name NTN3, Netrin-2-Like Protein, NTN2L
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CRPARGSYRISLKK, corresponding to amino acid residues 445 - 458 of mouse Netrin-3 (Accession Q9R1A3). Secreted.
Accession (Uniprot) Number Q9R1A3
Gene ID 18209
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – identical; Human – 13 out of 14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*, n*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    1-2. Anti-Netrin-3 Antibody (#ANR-123), (1:400).
    3-4. Anti-Netrin-3 Antibody, preincubated with Netrin-3 Blocking Peptide (BLP-NR123).
  • Western blot analysis of human U-87 MG glioma cell line lysates (lanes 1 and 3) and human SH-SY5Y neuroblastoma cell line lysates (lanes 2 and 4):
    Western blot analysis of human U-87 MG glioma cell line lysates (lanes 1 and 3) and human SH-SY5Y neuroblastoma cell line lysates (lanes 2 and 4):
    1-2. Anti-Netrin-3 Antibody (#ANR-123), (1:200).
    3-4. Anti-Netrin-3 Antibody, preincubated with Netrin-3 Blocking Peptide (BLP-NR123).
Immunohistochemistry
  • Expression of Netrin-3 in rat spinal cord.
    Expression of Netrin-3 in rat spinal cord.
    Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-Netrin-3 Antibody (#ANR-123), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the ventral horn region showed Netrin-3 immunoreactivity (green) in neuronal profiles (arrows). B. Pre-incubation of the antibody with Netrin-3 Blocking Peptide (BLP-NR123), suppressed staining. Cell nuclei are stained with DAPI (blue).
References
  1. Sun. J., et al (2011) Development. 138, 2153-2169.
  2. Rajasekharan, S, et al (2009) Genome. Biol. 10, 239.
  3. Bruikman, C. et al (2019) J. Nucleic Acids 2019, 9.
Scientific background Netrins are a family of highly conserved proteins responsible for axon guidance and cell movement throughout neural development. Netrins can be divided into secreted netrins (netrin 1,3,4 and 5) and membrane-tethered glycophosphatidylinositol (GPI)-linked netrins (netrin G1 and G2)1.

Secreted netrins carry their function via interaction with several receptors that include the deleted in colorectal (DCC) family, and the uncoordinated-5 (UNC5-A through UNC5-D) family2.

Netrin-3 was discovered in 1997 using sequence homology searching of netrin-2. Similarly to other netrins, Netrin-3 plays an important role in the development of the nervous system. Netrin-3 structure consist of a laminin-like domain located on the N-terminal, three epidermal growth factors like repeats (EGF), and a C-terminal netrin-like domain (NTR). Mutations in NTN3, the gene encoding netrin-3 was found to be associated with the development of several carcinomas3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 31/08/2021

Anti-Netrin-3 Antibody (#ANR-123) is a highly specific antibody directed against an epitope of the mouse Netrin-3 protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Netrin-3 from rat, mouse and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

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