Anti-Neurexin 1α (extracellular)-ATTO-488 Antibody

Neurexin I-alpha, Nrxn1
    Cat #: ANR-031-AG
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)KPRHQKDAKHPQMIK, corresponding to amino acid residues 546-560 of rat Neurexin 1α (Accession Q63372). Extracellular, N-terminus.
    Accession (Uniprot) Number Q63372
    Gene ID 60391
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANR-031), and immunohistochemistry (labeled antibody).
    Applications: ih
    May also work in: ic, lci
    Immunohistochemistry
    Immuno-colocalization of Neurexin 1α and GABA(A) ε Receptor in mouse cerebellum
    Immunohistochemical staining of rat cerebellum frozen section using Anti-Neurexin 1α (extracellular)-ATTO-488 Antibody (#ANR-031-AG) and Anti-GABA(A) ε Receptor (extracellular)-ATTO-633 Antibody (#AGA-015-FR). A. Nrxn1α staining (green). B. GABA(A) ε Receptor staining (purple). C. Merge of the two images suggests extensive co-localization in Purkinje cells (arrow).
    References
    1. Ichtchenko, K. et al. (1995) Cell 81, 435.
    2. Mosedale, M. et al. (2012) J. Biol. Chem287, 6350.
    3. Ullrich, B. et al. (1995) Neuron 14, 497.
    4. Zhang, C. et al. (2010) Neuron 66, 403.
    5. Laarakker, M.C. et al. (2010) PLoS One 7, e31503.
    Scientific background

    Neurexins (NRXNs) are a family of transmembrane, synaptic adhesion molecules. NRXNS were identified as receptors for α-latrotoxin, a presynaptic toxin that triggers massive neurotransmitter release1. Neurexins are largely presynaptic proteins that form a trans-synaptic cell-adhesion complex with postsynaptic neuroligins2. They are encoded by three genes (NRXN1, NRXN2 and NRXN3), each using an upstream promoter to produce the longer α-isoform (α-NRXNs) and a downstream promoter to generate a shorter β-isoform (β- NRXNs).

    The α- and β-isoforms of each neurexin are single-pass transmembrane proteins maintaining identical transmembrane and intracellular domains but having distinct extracellular domains. NRXNs in neurons localize to the presynaptic membrane and bind trans-synaptically to postsynaptic adhesion molecules and receptors3. Neurexins are expressed in all neurons, and are subject to extensive alternative splicing, generating >1,000 splice variants, some of which exhibit highly regulated developmental and spatial expression patterns4

    The extracellular domain of neurexin 1α (NRXN1α) is composed of three neurexin repeats (I, II, and III), which each contains the modules LNS-EGF-LNS. NRXN1α binds endogenous ligands including neuroligins, LRRTM family members, neurexophilin, α-dystroglycan, and GABA(A) receptors.

    NRXN1α gene mutations have been identified in neuropsychiatric diseases including Schizophrenia and Autism Spectrum Disorder5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 26/11/2018

    Anti-Neurexin 1α (extracellular) Antibody (#ANR-031) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot and live cell imaging applications. It has been designed to recognize Nrxn1α from rat, mouse and human samples.

    Anti-Neurexin 1α (extracellular)-ATTO-488 Antibody (#ANR-031-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Neurexin 1α (extracellular)-ATTO-488 Antibody has been tested in immunohistochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use